저온에서 벼의 발아율 및 발아속도 관련 양적형질 유전자좌(QTL) 분석 = QTL Analysis of Germination Rate and Germination Coefficient of Velocity under Low Temperature in Rice

다국어 초록 (Multilingual Abstract)

As rice originates from tropical regions, low temperature stress during the germination stage in temperate regions leads to serious problems inhibiting germination and seedling establishment. Identifying and characterizing quantitative trait loci (QTLs) for low-temperature germination (LTG) resistance help accelerate the development of rice cultivars with LTG tolerance.
In this study, we identified QTLs for LTG tolerance (qLTG5, qLTG9) and germination coefficient of velocity under optimal conditions (OGCV) (qOGCV7, qOGCV9) using 129 recombinant inbred lines (RILs) derived from the cross between a low-temperature sensitive line Milyang23 and a low-temperature tolerant variety Gihobyeo. qLTG9 and qOGCV9 were detected at the same location on chromosome 9. At both LTG QTLs (qLTG5 and qLTG9), the alleles for LTG tolerance were contributed by the japonica variety Gihobyeo. At qOGCV7 and qOGCV9, the alleles for low temperature tolerance were derived from Milyang23 and Gihobyeo, respectively. The RILs with desirable alleles at two or more QTLs, i.e., GroupVII: qLTG5+qLTG9 (qOGCV9) and GroupVIII: qLTG5+qOGCV7+qLTG9 (qOGCV9), showed stable tolerance under low-temperature stress. Our results are expected to contribute to the improvement of tolerance to low-temperature and anaerobic stress in japonica rice, which would lead to the wide adoption of direct-seeding practices.

국문 초록 (Abstract)

자포니카 벼의 저온 스트레스 내성 증진을 위하여, RIL 계통을 이용하여 저온 스트레스 내성 QTL을 탐색하였다. 이를통하여 (1) 5, 9번 염색체에서 저온발아에 관련한 ‘기호벼’ 유래 QTL, qLTG5와 qLTG9를 확인하였으며, 7, 9번 염색체에서 저온 발아속도에 관련한 ‘밀양23호’ 및 ‘기호벼’ 유래 QTL, qOGCV7, qOGCV9를 확인하였다. (2) Duncan 검정결과, 그룹Ⅶ [qLTG5+qLTG9 (qOGCV9)], 그룹Ⅷ [qLTG5+ qOGCV7+qLTG9 (qOGCV9)]의 계통들이 저온 스트레스에내성이 있는 것으로 확인 되었다. (3) 최근 발표된 RIL 집단 담수내성 계통과 비교한 결과, 저온 스트레스에도 내성이 있으면서 담수발아에도 내성이 있는 것으로 확인된 총2개의 유망 유전자원을 선발하였다.
본 연구의 결과를 통해 저온 및 혐기 관련 QTL의 집적은벼의 저온에서의 발아 및 초기 입모율을 높여 저온스트레스 내성 개선에 도움이 되는 것으로 판단 되었으며, 선발된유망 계통은 향후 직파재배 품종 육성에 유용한 유전자원으로 활용되어 직파재배의 안정성 증대에 기여할 것으로기대된다.

참고문헌 (Reference)

1 지현소, "벼의 밀양23호/기호벼 재조합자식 유전집단을 이용한 PCR 기반 DNA 마커들로 구성된 분자유전자지도 작성" 한국육종학회 44 (44): 273-281, 2012

2 고종철, "벼 직파 품종육성을 위한 기존 품종의 저온발아성 분석" 한국국제농업개발학회 23 (23): 89-94, 2011

3 정종민, "담수 직파에서 산소 및 온도조건에 따른 혐기발아 내성 자원과 국내 직파 적응성벼 품종의 입모특성 비교" 한국작물학회 64 (64): 287-298, 2019

4 손지영, "국내 육성 주요 벼 품종의 저장기간과 발아온도에 따른 발아율과 발아속도 변화" 한국국제농업개발학회 26 (26): 440-446, 2014

5 Li, L., "qLTG-9, a stable quantitative trait locus for low-temperature germination in rice (Oryza sativa L.)" 126 : 2313-2322, 2013

6 Najeeb, S., "Y. L. Pang, J. Zilhas, V. Murugaiyan, L. R. Vemireddy, and Z. Li. 2020. Identification of maineffect quantitative trait loci (QTLs) for low-temperature stress tolerance germination- and early seedling vigor-related traits in rice (Oryza sativa L.)" 40 : 10-, 2020

7 Causse, M. A., "Saturated molecular map of the rice genome based on an interspecific backcross population" 138 : 1251-1274, 1994

8 Jiang, L., "Quantitative trait loci and epistatic analysis of seed anoxia germinability in rice(Oryza sativa)" 11 : 238-244, 2004

9 Teng, S., "QTL analysis of rice low temperature germinability" 46 : 1800-1804, 2001

10 Jinhee Kim, "QTL Analysis for Tolerance to Anaerobic Germination in Japonica Rice (Oryza sativa L.)" Korean Society of Breeding Science 51 (51): 415-423, 2019

11 Wang, X., "Natural variation reveals that OsSAP16 controls low-Temperature germination in rice" 69 : 413-421, 2018

12 Fujino, K., "Molecular identification of a major quantitative trait locus, qLTG3-1, controlling low-temperature germinability in rice" 105 : 12623-12628, 2008

13 Thomas, B. R, "Metabolite signals regulate gene expression and source/sink relations in cereal seedlings" 106 : 1235-, 1994

14 Redoña, E. D., "Mapping quantitative trait loci for seedling vigor in rice using RFLPs" 92 : 395-402, 1996

15 Miura, K., "Mapping quantitative trait loci controlling low temperature germinability in rice (Oryza sativa L.)" 51 : 293-299, 2001

16 Jiang, N., "Mapping qtl for seed germinability under low temperature using a new high-density genetic map of rice" 8 : 1-9, 2017

17 Fujino, K., "Mapping of quantitative trait loci controlling low-temperature germinability in rice (Oryza sativa L.). Theor" 108 : 794-799, 2004

18 Andaya, V. C., "Mapping of QTLs associated with cold tolerance during the vegetative stage in rice" 54 : 2579-2585, 2003

19 Zhang, L., "Interactions between markers can be caused by the dominance effect of quantitative trait loci" 180 : 1177-1190, 2008

20 Hou, M. Y., "Inheritance and QTL mapping of low temperature germinability in rice (Oryza sativa L.)" 31 : 701-706, 2004

21 Alhamdam, A. M., "Influence of Storage Conditions on Seed Quality and Longevity of Four Vegetable Crops" 11 : 353-359, 2011

22 Suh, J. P., "Identification and analysis of QTLs controlling cold tolerance at the reproductive stage and validation of effective QTLs in cold-tolerant genotypes of rice (Oryza sativa L.)" 120 : 985-995, 2010

23 Jiang, S., "Genetic dissection of germinability under low temperature by building a resequencing linkage map in japonica rice" 21 : 1284-, 2020

24 Shakiba, E., "Genetic architecture of cold tolerance in rice(Oryza sativa)determined through high resolution genome-wide analysis" 12 : 1-22, 2017

25 Han, L. Z., "Genetic and QTL Analysis for Low-Temperature Vigor of Germination in Rice" 33 : 998-1006, 2006

26 Churchill, G. A., "Empirical threshold values for quantitative trait mapping" 138 : 963-971, 1994

27 Wang, W., "Effects of pre-sowing seed treatments on establishment of dry direct-seeded early rice under chilling stress" 8 : 2016

28 Pradhan, B. K., "Effect of storage conditions and storage periods on seed germination in eleven populations of Swertia chirayita : A critically endangered medicinal herb in Himalaya" 2012 : 1-9, 2012

29 Cho, Y. G., "Development of RFLP framework map of rice(Oryza sativa L. )using recombinant inbred population derived from Milyang23/Gihobyeo cross" 30 : 289-297, 1998

30 Lin, J., "Detection of QTL for Cold Tolerance at Bud Bursting Stage Using Chromosome Segment Substitution Lines in Rice(Oryza sativa)" 18 : 71-74, 2011

31 Lincoln, S. E., "Constructing Genetic Linkage Maps with MAPMAKER/EXP Version 3.0:A Tutorial and Reference Manual"

32 Ye, C., "Cold tolerance in rice varieties at different growth stages" 60 : 328-338, 2009

33 Jiang, L., "Analysis of QTLs for seed low temperature germinability and anoxia germinability in rice (Oryza sativa L.)" 98 : 68-75, 2006

34 Wade, M. J., "Alternative definitions of epistasis : Dependence and interaction" 16 : 498-504, 2001

35 Wright, M. H., "ALCHEMY : A reliable method for automated SNP genotype calling for small batch sizes and highly homozygous populations" 26 : 2952-2960, 2010

36 Zhao, J., "A novel functional gene associated with cold tolerance at the seedling stage in rice" 15 : 1141-1148, 2017

37 Lou, Q., "A major QTL associated with cold tolerance at seedling stage in rice(Oryza sativa L. )" 158 : 87-94, 2007