Topical Prostaglandin Analogue Drugs Inhibit Adipocyte Differentiation

다국어 초록 (Multilingual Abstract)

Purpose: To investigate the effects of topical prostaglandin analogue drugs on the differentiation of adipocytes.
Methods: Differentiation of 3T3-L1 preadipocytes was induced with isobutylmethylxanthine, dexamethasone,and insulin. 3T3-L1 cells were exposed to 0.008, 0.08, 0.2 mM of latanoprost and travoprost. Reverse transcriptionpolymerase chain reaction for mRNA expression of lipoprotein lipase and peroxisome proliferator-activatedreceptor γ 2 (PPARγ2), and glycerol-3-phosphate dehydrogenase (G3PDH) assays were performed toexamine the effects on early and late differentiation, respectively. Also, glycerol assays were done to evaluatethe effect of prostaglandin analogues on lipolysis after differentiation.
Results: Both prostaglandin analogues inhibited differentiation of preadipocytes. Topical prostaglandin analoguessignificantly decreased G3PDH activity, a marker of late differentiation. However, topical prostaglandinanalogues did not change mRNA expressions of lipoprotein lipase and PPARg2, markers of early differentiation.
The activities of the early markers of differentiation were not changed significantly before and aftergrowth arrest. Compared to latanoprost, travoprost decreased G3PDH activity more significantly (p < 0.05).
Both prostaglandin analogues did not affect the lipolysis of differentiated adipocytes (p > 0.05).
Conclusions: Prostaglandin analogues display an inhibitory effect on the differentiation of adipocytes when thecells start to differentiate especially in the late stage of differentiation. Thus, commercial topical prostaglandinanalogues may decrease the fat contents of eyelids.

참고문헌 (Reference)

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