고추(Capsicum annuum) callis와 검은 무늬병 유발균의 elicitor를 이용한 항균물질 생산 = Production of Antimicrobial agents by Co-culture Elicitor of Black rot's disease and Callus of Capsicum annuum

다국어 초록 (Multilingual Abstract)

We described the effects of antimicrobial activity on a produced material by the co-culture of elicitor inducing black rot's disease and callus of Capsicum annuum that callus was induced in MS medium containing IAA 0.5 mg/ml and kinetin 2.0 mg/ml. the bacterial elicitor used 0.5% Yeast extract and the fungal elicitor made by Alternaria alternata KACC 40019 with PDB medium. In order to produce antimicrobial agent protective balck rot's disease, we co-cultured the fungal elicitor or bacterial elicitor with the red pepper callus tissue for 7days repectively. The result, an antifungal agent was produced extracellular materials into medium during co-culture red pepper callus with the fungal elicitor. The antifungal activity of the extracellular material was showed 23% inhibition rate on Alternaria alternata KACC 40019 on the other hand the intracellular material was showed 2% inhibition rate. A chitinase activity of extracellular material was also showed higher value than a chitinase of the intracellular material. The extracellular material produced by elicitor was had more 15% of andtibacterial activity than the intracellular material. As a result, a biotic stress as a fungal elicitor and a bacterial elicitor brings a defence system in the red pepper callus and induce to synthesis in extracellular material an antimicrobial agent as a chitinase and phytoalexin.

목차 (Table of Contents)

• Ⅰ. 서론 2
• Ⅱ. 재료 및 방법 2
• 1. 실험 재료 2
• 2. 실험 방법 2
• 1) 종자 살균 및 발아 유도 2
• 2) calius culture 3
• 3) biotic elicitor 제조 3
• (1) fungal elicitor 제조 3
• (2) bacterial elicitor 제조 3
• 4) 고추callus와 biotic elicitor와의 co-culture 3
• (1) 고추callus와 elicitor의 co-culture 3
• (2) 고추callus와 bacterial elicitor의 co-culture 3
• 5) 고추 callus로부터 antimicrobial agent cncnf 3
• 6) chitinase(EC 3. 2. 1. 14) activity 검정 3
• 7) Antifungal activity 3
• 8) antibacterial activity 4
• Ⅲ. 결과 및 고찰 4
• 1. 고추종자의 발아 및 callus 분화 효율 4
• 2. 고추 callus가 생산한 항진균 물질 검정 4
• 1) chitinase 활성 검정 4
• 2) 검은 무늬병 유발 곰팡이에 대한 항진균 활성 검정 5
• 3. 고추 callus가 생산한 항균 물질 검정 5
• Ⅳ. 결론 7
• 참고문헌 8