본 연구에서는 스테비아는 이용성이 높은 식물임에도 불구하고 diterpene 성분에 대한 생리활성이 과학적으로 주로 연구된 바 없어 active-guided fractionation 방법을 이용하여 항염증 효과를 나타...
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https://www.riss.kr/link?id=A60111949
2012
Korean
KCI등재,SCOPUS
학술저널
456-461(6쪽)
6
0
상세조회0
다운로드국문 초록 (Abstract)
본 연구에서는 스테비아는 이용성이 높은 식물임에도 불구하고 diterpene 성분에 대한 생리활성이 과학적으로 주로 연구된 바 없어 active-guided fractionation 방법을 이용하여 항염증 효과를 나타...
본 연구에서는 스테비아는 이용성이 높은 식물임에도 불구하고 diterpene 성분에 대한 생리활성이 과학적으로 주로 연구된 바 없어 active-guided fractionation 방법을 이용하여 항염증 효과를 나타내는 지표화합물을 규명하기 위해 실리카겔 칼럼크로마토그래피 방법을 이용하여 분리 및 정제한 후, <SUP>1</SUP>H와 <SUP>13</SUP>C-NMR, COSY, DEPT, HMQC, HMBC spectrum(500 MHz, CDCl3), MS, IR 분석을 통하여 분자량 322의 astroinulin임을 구조 동정하였다. 특히 HMBC spectrum을 통해 분리된 화합물이 decalin system에서 C-9에 3-me-thylpenta-2,4-dienyl 치환체를 가지고 있음을 알 수 있었다. 분리된 astroinulin을 RAW264.7 세포에 2.5, 5, 10 μg/mL 처리한 결과 농도 의존적으로 NO의 생성을 억제하였다. 마찬가지로 iNOS protein의 발현에서도 농도 의존적으로 저해하였으며, 화합물을 2.5, 5, 10 μg/mL 처리한 결과 각각 11.4, 26.8, 45.1% 감소하였다. Astroinulin 화합물을 10 μg/mL의 낮은 농도에서 NO와 iNOS를 각각 67.9, 45.1%를 저해하는 결과를 나타내었다. 이와 같은 결과는 스테비아에서 분리된 astroinulin 화합물은 RAW264.7 세포에서 LPS에 의해 유도된 NO와 iNOS 단백질을 유의적으로 억제하는 것으로 확인되어 이를 이용한 기능성식품 및 의약품 개발 가능성을 지닌 약용 식물자원인 것으로 판단된다.
다국어 초록 (Multilingual Abstract)
The leaves of Stevia rebaudiana are well-known in Japan, Korea, and China as a natural sweetener. Medicinal uses of this plant originated in Paraguay and Brazil in the form of aqueous decoctions of the leaves used as a contraceptive agent and for the ...
The leaves of Stevia rebaudiana are well-known in Japan, Korea, and China as a natural sweetener. Medicinal uses of this plant originated in Paraguay and Brazil in the form of aqueous decoctions of the leaves used as a contraceptive agent and for the treatment of hyperglycemia. In the present study, the antioxidant, anti-hyper-tension, and anti-inflammatory activities of S. rebaudiana extracts are investigated for their use in food. The biologically-active compound was isolated and purified from S. rebaudiana. The isolated compound was identified as austroinulin (C20H34O3; molecular weight 322) by mass, IR spectrophotometry, 1D, and 2D-NMR. Austroinulin was characterized as a diterpenoid possessing a 3-methylpenta-2,4-dienyl at C-9. When subjected to an inflammatory mediator inhibitory assay from lipopolysaccharide (LPS)-activated macrophages, the austroinulin inhibited the enhanced production of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) expression (10 μg/mL=67.9 and 45.1%, respectively). This was significant and dose-dependent. The results suggest that austroinulin from S. rebaudiana inhibited the NO and iNOS in RAW 264.7 cells.
참고문헌 (Reference)
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1 Suh SJ, "The naturally occurring biflavonoid, ochnaflavone, inhibits LPS-induced iNOS expression, which is mediated by ERK1/2 via NF-κB regulation, in RAW264.7 cells" Academic Press 447 (447): 136-146, 200603
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4 Oshima Y, "Sterebins A, B, C and D, bisnorditerpenoids of Stevia rebaudiana leaves" 42 : 6443-6446, 1986
5 Metzger BT, "Purple carrot (Daucus carota L.) polyacetylenes decrease lipopolysaccharide- induced expression of inflammatory proteins in macrophage and endothelial cells" 56 : 3554-3560, 2008
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7 Sholichin M, "Labdane-type diterpenes from Stevia rebaudiana" 19 : 326-327, 1980
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고지방식이로 유도한 지방간 마우스에서 기장 첨가식이가 지방간 및 인슐린 저항성에 미치는 영향
다이옥신계 TCDD(2,3,7,8-Tetrachlorodibenzo-p-dioxin)에 노출된 흰쥐의 지질대사 및 면역관련 인자에 대하여 키토산 올리고머의 분자량별 섭취효과
마우스 피부암에 대한 장뇌삼과 인삼의 특이적 항암 효능
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2023 | 평가예정 | 해외DB학술지평가 신청대상 (해외등재 학술지 평가) | |
2020-01-01 | 평가 | 등재학술지 유지 (해외등재 학술지 평가) | |
2014-06-24 | 학회명변경 | 한글명 : 한국식품영양과학회지 -> 한국식품영양과학회영문명 : Journal of the Korean Society of Food Science and Nutrition -> The Korean Society of Food Science and Nutrition | |
2014-04-02 | 학회명변경 | 한글명 : 한국식품영양과학회 -> 한국식품영양과학회지영문명 : 미등록 -> Journal of the Korean Society of Food Science and Nutrition | |
2011-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2009-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2007-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2005-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2002-07-01 | 평가 | 등재학술지 선정 (등재후보2차) | |
2000-01-01 | 평가 | 등재후보학술지 선정 (신규평가) |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 1.03 | 1.03 | 1.13 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
1.18 | 1.2 | 1.993 | 0.21 |