백강균의 분생자병속 형성 조건 = Conditions for Formation of Synnemata from Beauveria bassiana

국문 초록 (Abstract)

백강균(Beauveria bassiana)의 분생자병속 형성 조건 구명을 위해 본 실험을 수행하였다. 균은 제주도 설악산 일대에서 치사한 참매미로부터 분리되었다. 분생자병속 형성 유도 배지는 현미와 누에번데기를 원료로 하여 90:10 비율로 조제한 경우가 가장 효과적이었으며, 기타 배지 상에서는 분생자병속 발생이 관찰되지 않았다. 분생자병속은 공기 유입량이 최소일 때 형성이 효과적이었으며, 분생자병속 발생의 최적 온도는 $20^{\circ}C$, 배지 수분함량은 60% 및 조도 300 lux이었다. 또한, 증식에 활용한 종균은 액체배양균이 무성포자보다 분생자병속 형성에 더욱 효과적이었다.

다국어 초록 (Multilingual Abstract)

This study was conducted to investigate optimal conditions for formation of synnemata from Beauveria bassiana. The strain of B. bassiana was isolated from a host of Oncotympana fuscata in Halla mountain of Jeju island. The yield of synnemata was the highest at application of brown rice (90%) and silkworm pupae (10%) media. On the other hand, the other media did not result in formation of synnemata. The highest formation of synnemata was achieved at conditions of moderate air inflow and $20^{\circ}C$. The optimal moisture and light intensity for formation of synnemata were 60% and 300 lux, respectively. In addition, inoculation of the liquid spawn resulted in higher yield than that of asexual spore.

참고문헌 (Reference)

1 Ha, N.G., "눈꽃동충하초의 배양적 특성 및 생리활성" 47 : 12-17, 2005

2 Choi, I.Y., "The production of artificial fruiting body of Paecilomyces japonica" 27 : 87-93, 1999

3 Pen, X., "The cultivation of C. militaris fruitbody on artificial media and the determination of SOD activity" 2 : 25-28, 1995

4 Choi, I.Y., "The condition of production of artificial fruiting body of Cordyceps militaris" 27 : 243-248, 1999

5 Basith, M., "Studies on the production of perihelial stigmata by Cordyceps militaris in artificial culture" 46 : 473-480, 1968

6 Kobayasi, Y., "Keys to the taxa of the genera Cordyceps and Torrubiella" 23 : 329-364, 1982

7 Jianzhe, Y., "Icons of medicinal fungi from China" Science Press 575-, 1989

8 Kobayasi, Y., "Iconography of vegetable wasps and plant worms" Hoikusha Publishing Company Ltd. 280-, 1983

9 Carilli, A., "Growth and sporulation of Cordyceps militaris (Linn. Ex Fr.) Link in submerged culture" 68 : 237-243, 1977

10 Shah, P.A., "Entomopathogenic fungi as biological control agents" 61 : 413-423, 2003

11 Hubbel, H. R., "Differential antiproliferative actions of 2', 5' oligo a trimer core and its cordycepin analogue on human tumor cells" 36 : 389-394, 1985

12 Hawksworth, D. L., "Dictionary of the fungi" CAB international 51-, 1995

13 Nam, S.H., "Cultural condition and morphological characteristics of Paecilomyces japonica for the artificial cultivation" 41 : 36-40, 1999

14 Cunningham, K. G., "Cordycepin, metabolic product isolated from cultures of Cordyceps militaris (Linn.) Link" 166 : 949-, 1950

15 Cunningham, K. G., "Cordycepin, a metabolic product from cultures of Cordyceps militaris (Linn.) Link. Part I. Isolation and characterization" 2299-2300, 1951

16 Samson, R.A., "Atlas of Entomopathogenic Fungi" Springer 1988

17 Ohmori, T., "Antitumor activity of protein-bound polysaccharide from Cordyceps ophioglossoides in mice" 77 : 1256-1263, 1986