Chitosan-coated Ferrite (Fe3O4) Nanoparticles as a T2 Contrast Agent for Magnetic Resonance Imaging

다국어 초록 (Multilingual Abstract)

Iron oxide (Fe3O4) nanoparticles coated with biocompatible chitosan were synthesized for use as an MRI (magnetic resonance imaging) contrast agent. The coating was performed simultaneously with the synthesis of the ferrite nanoparticles. A dynamic light-scattering spectrometer (DLS)and a transmission electron microscope (TEM) were used to measure the average diameter of the coated nanoparticles, which was 67.0 nm. Fourier transform infrared (FT-IR) measurements showed strong bonding of the chitosan molecules to the surfaces of the ferrite nanoparticles. The spin-lattice (T1) and the spin-spin (T2) relaxation times of the nuclear spins (hydrogen protons) in aqueous solutions of various concentrations of coated ferrite nanoparticles were determined using a nuclear magnetic resonance (NMR) spectrometer. Using these data, we found that the T1 and the T2 relaxivities of the nuclear spins in aqueous solutions of ferrite nanoparticles were 0.00291 and 0.0691 ppm−1sec−1, respectively. In particular, the value of the T2 relaxivity was much larger than that of the commercial contrast agent GD-DTPA (gadolinium diethylenetriamine penta-acetic acid).
A 31.7% intensity loss in the T2 image of a rabbit liver was observed after injecting the aqueous solution of coated nanoparticles into the rabbit, which shows that our coated ferrite nanoparticles can be used as a T2 MRI contrast agent.

참고문헌 (Reference)

1 E. X. Wu, 17 : 478-, 2004

2 D. Zhao, 477 : 739-, 2009

3 T. G. Loflin, 157 : 457-, 1985

4 B. Misselwitz, 12 : 128-, 2001

5 H. Weinmann, 22 : 233-, 1991

6 Y. Okuhata, 37 : 121-, 1999

7 I. Rhee, 261 : 410-, 2003

8 E. H. Kim, 289 : 328-, 2005

9 S.-W. Hong, 4 : 27-, 2000

10 A. Borel, 110 : 12434-, 2006

11 Y. Hu, 23 : 3193-, 2002

12 Q. Chen, 5 : 993-, 2005

13 W. B. Tan, 75A : 56-, 2005

14 Y. Chen, 10 : 621-, 2003

15 K. Nishimura, 91 : 8555-, 2002

16 K. Van de Velde, 58 : 409-, 2004

17 P. Kolhe, 4 : 173-, 2003

18 Y. Sun, 20 : 135102-, 2009

19 S. A. Agnihotri, 100 : 5-, 2004

20 R. B. Lauffer, 87 : 901-, 1987

21 "http://www.amagpharma.com"

22 Sungwook Hong, "Signal Loss in the T2-Weighted Magnetic Resonance Images by Using an USPIO-Particle Contrast Agent" 한국물리학회 51 (51): 1453-1456, 2007

23 Sungwook Hong, "Relaxation Rate of Hydrogen Protons of Water Molecules in an Aqueous Solution of Dextran Coated Ferrite Nanoparticles" 한국물리학회 49 (49): 676-678, 2006

24 P. Mansfield, "NMR Imaging in Biomedicine" Academic Press, Inc. 17-, 1986

25 Ilsu Rhee, "Anomalous Behavior of T2 Relaxation Time of Hydrogen Protons in Highly Concentrated Iron Ferrite Nanoparticle Solution" 한국물리학회 45 (45): 1037-1039, 2004