태생 전 임신 15일째 Methylazoxymethanol에 노출된 쥐의 수면각성주기 이상 및 신경생리학적 이상 = Sleep-Wake Cycle and Neurophysiological Abnormalities in Adult Rats after Prenatal Exposure to Methylazoxymethanol Acetate at Gestational Day 15

국문 초록 (Abstract)

뇌 발육이상 중 신경이주장애(neuronal migration disorder)에 의해 나타나는 비정상적인 피질 층 구조는 난치성 간질과 발달장애, 정신지체, 행동 및 정서장애 등과 관련 있다고 알려져 있다. 본 연구에서는 태생기 MAM에 노출시켜 신경이주장애를 유발시킨 쥐에서 수면 이상과 뇌파 이상을 수면-각성 상태의 주기의 관찰과 각 상태에서 뇌파의 정량적 기술을 이용하여 살펴보았다. 그리고 신경이주장애와 관련된 질환에 동반되어 생길 수 있는 수면 이상 및 뇌파 이상을 고찰하였다.
MAM 노출 동물에서 역설수면 기간이 정상 동물과 비교할 때 유의하게 감소되었으나 다른 수면 각성 상태의 기간들의 유의한 변화는 없었다. 전체 7시간 관찰(10：30-17：30) 동안 MAM 노출 쥐에서는 전두피질, 중앙피질, 두정피질에서 저명한 진폭 스펙트럼의 변화가 관찰되었다. 특정 뇌영역의 기능 상태를 나타내는 진폭분포스펙트럼 비교에서 모든 피질 영역의 쎄타 파가 MAM 노출 쥐에서 정상 쥐에서보다 감소되었다. 반대로 역설수면 동안 정상 쥐에서 두정부에서 저명한 쎄타 활동이 관찰되지만 MAM 노출 쥐에서는 진폭이 작고 빠른 쎄타파가 관찰되고, 정상 동물의 전두피질에서 쎄타 및 느린 주파수의 쎄타가 관찰되는 반면 MAM 노출 쥐에서는 알파 대역의 빠른 주파수의 방추형파가 기록되었다.
MAM 처리된 쥐들에서 나타나는 활동성의 증가와 수면 지연 등의 결과를 고려해 볼 때, 발달동안의 유발된 피질의 기형은 수면 각성의 특성을 변화시킬 뿐만 아니라 전반적인 기능의 이상을 가져옴을 시사한다.

다국어 초록 (Multilingual Abstract)

Several neuropsychiatric disorders, including intractable epilepsy, mental retardation or schizophrenia, are consequences of cortical malformation. We examined the sleep-wake profiles and the electroencephalographic(EEG) abnormalities in the adult rats exposed to methylazoxymethanol acetate(MAM) at embryonic day 15 whether cortical disorganization could affect sleep-wake cycle and neurophysiological characteristics.
In the MAM-exposed rats, only the duration of the paradoxical sleep(PS) decreased significantly while the durations of other sleep-wake states did not differ from the normal control group. The amplitude spectrum of the 7-hour EEG recordings(10：30-17：30) of the frontal, central and parietal cortices of the MAM-exposed group was compared with that of the control group. A significant amplitude decrease was seen in high delta and theta bands of the frontal EEG, in the theta band of the central EEG and in from the delta to low beta ranges of the parietal EEG. The relative amplitude distribution spectrum, which represents a specific functional state of the brain, of the MAM-exposed rats was compared with that of the control group. Significant decrease of the percentage of amplitude was seen in the theta band of the frontal, central and parietal EEG. Conversely, significant increase was seen in the beta band of the central EEG and in the beta and low gamma bands of the parietal EEG. The peak frequency of the theta wave, which is prominent in the parietal EEG, was higher in MAM-exposed rats than in the control group. In visual inspection of the EEG trace of the MAM-treated rat, spindle rhythms of the beta range were prominent during the transition to PS, the PS and even during the slow wave sleep and delta wave amplitude was smaller and the theta frequency was faster as compared with the control group.
These observations correspond to the changed in the percentage of amplitude in the relative distribution spectrum. Taken together, the findings suggest that the cortical malformation during development produces an alteration in the global functional state indicated by the EEG changes as well as alters the sleep-wakefulness characteristics.

목차 (Table of Contents)

• 서론
• 방법
• 결과
• 고찰
• 요약
• 참고문헌
• 사진부도

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