Yersinia enterocolitica O:1,2,3 군과 O:3 군의 균체응집항원과 침강항원의 특이성 = Specificity of O-agglutinogen and Precipitinogen of Yersinia enterocolitica Serogroups O:1,2,3 and O:3

다국어 초록 (Multilingual Abstract)

By means of bacterial agglutination, agar gel-diffusion, rocket and two-dimentional immunoelectrophoresis, antigenic relationships of bacterial agglutinogens and precipitinogens between three strains of serogroup 0: 1,2,3 and two strains of serogroup 0:3 of Yersinia enterocolitica were studied.
The results were summarized as follows:
1. Crossed bacterial agglutination reactions between strains of 0:1,2,3 and 0:3 to homologous and heterologous rabbit immune sera showed so close relations that strains of two serogroups were not differentiated one another by agglutination test using rabbit anti -0:1, -0:2 and -0:3 sera.
2. Bacterial antigens of 0:1, 2 ,3 cross-reacted with both anti-0:1 serum absorbed with 0:2 and 0:3 groups and anti -0:2 serum absorbed with 0:1 and 0:3 groups, whereas the bacterial antigens of 0:3 group reacted with only absorbed anti-0:1 serum.
3. Out of three bacterial soluble antigens extracted with 0.3% phenol-phosphate buffered saline (PBS), 0.1% Triton X-100-phosphate buffer and 10% trichloroacetic acid solution, the precipitinogen of PBS extract was most highly reactive and stable up to 121˚C for 30 min. The precipitinogen, however, heated at 60˚C for 30 min was more reactive. The heat-stable surface antigen of 0:1,2,3 group was antigenically identical with the antigen of 0:3 group.
4. Fused rocket immunoelectrophoresis revealed that the antigens of 0:1,2,3 and 0:3 groups had one to two common antigens which had a rapider mobility than group specific antigens.
5. By crossed immunoelectrophoresis, the precipitinogens of 0:1,2,3 group had four to five antigens having the relative electrophoretic mobility of 0, 0.15 - 0.18, 0.41, 0.47 -0.5 and 0.58, whereas the precipitinogens of 0:3 group had three antigens having the relative electrophoretic mobility of 0, 0.15 - 0.18 and 0.47 - 0.5.
These results indicated that although the strains of 0:1,2,3, group would differ in their subagglutinogens from those of 0:3 group they belonged to 0:3 group by their common 0 group-specific precipitinogens.