Label-free and multiplex detection of Alzheimer’s disease biomarkers using gold nanoplasmonic biosensor based on LSPR with colorimetric assay = Label-free and multiplex detection of Alzheimer’s disease biomarkers using gold nanoplasmonic biosensor based on LSPR with colorimetric assay

다국어 초록 (Multilingual Abstract)

Alzheimer’s disease (AD) is a neurodegenerative disease associated with the loss of nerve cell in areas of the brain. To diagnose AD on early pathological stage, many AD studies focus on the blood-based diagnostic test which is easy to obtain the sample and reflects a change of biomarkers from CSF due to the drawbacks of common tests. Among the rich sources in blood, Amyloid beta 1-42, Amyloid beta 1-40 and Tau protein, which are closely associated with key mechanism of AD pathology occurring in the brain, are able to be supportive early screening and diagnostic tools. Here, we developed the multi-detection biosensor of those markers based on Rayleigh scattering and colorimetric assay depend on plasmonic properties of AuNPs. Also, we utilized chaotropic agent which interfere the binding between biomarkers and other plasma protein to increase the detection limit. This is the first platform to detect AD biomarkers in blood, which can be used to diagnose AD directly in the future.