식용생물로부터 면역증강활성물질의 탐색 및 추출조건 = Screening and Extraction conditions of Materials Activating Immune System from Edible Plants

다국어 초록 (Multilingual Abstract)

This study is focused on the screening and extraction of a macrophage activating polysacchRIDES from edile plants. Abstracts of the results obtained from researches are as followes.
Among 20 kinds of plant extracts, Allium Tuberosum, Allium Monanthum, Amaranthus Acendens and Capsicum Annuum showed higher macrophage lysosomal enzyme activity than others. They were than 185% activity in 100㎍/mL concentration in comparison with control. Especially, the cold water extracts from Allium Tuberosum showed the most potent activity in dose-dependent fashion. During extraction, low molecular weight fraction was removed by using MtOH extraction of Allium Tuberosum. High molecular water soluble fraction was obtained by hot water extraction and the EtOH precipitation. Fraction AT-1 showing 218% macrophage lysosomal enzyme activity, consisted of 52.1% carbohydrate, 11.2% protein and 3.2% uronic acid.

목차 (Table of Contents)

• ABSTRACT
• Ⅰ. 서론
• Ⅱ. 재료 및 방법
• 1. 시료 및 실험동물
• 2. 일반성분 분석
• 3. 구성당 분석 : Gas liquid chromatorgraphy(GLC)
• 4. Macrophage 활성능 측정
• 5. 검색용 시료의 조제
• Ⅲ. 결과 및 고찰
• 1. 각종 시료로부터 macrophage 활성화 물질의 검색
• 2. 추출 횟수별 macrophage 활성 검색
• 3. Macrophage 활성 물질의 추출 및 그 특성
• 4. 조다당 획분 AT-1의 화학적 조성 및 구성당 검색
• Ⅳ. 결론
• 참고문헌