Genotoxicity and Skin Penetration of Silver Nanoparticles in Hairless Mice After Dermal Exposure = Genotoxicity and Skin Penetration of Silver Nanoparticles in Hairless Mice After Dermal Exposure

다국어 초록 (Multilingual Abstract)

Silver nanoparticles have been used for a variety of consumer products which may directly contact the skin. This study aims to investigate genotoxicity in vitro and skin penetration of silver (Ag) nanoparticles of varying sizes in male SKH1-hairless mice. Dorsal skin of six-week-old hairless mice was treated with 5-10, 100, and 160 nm silver nanoparticles at 1, 10, and 100 mg/kg body weight for 1 month. After dermal exposure of size-dependent silver nanoparticles for 4weeks, blood, urine and organ distribution of silver nanoparticles was measured by ICP-MS (inductively coupled plasma mass spectrometry) analysis. In case of 5-10nm silver nanoparticle treatment, silver concentrations were increased in a dosedependent manner at all measured organs and body fluid. Silver nanoparticles were absorbed through the skin and distributed in the body. The concentrations of silver nanoparticles were highest in the liver among various organs measured and followed by kidney, heart, and brain (liver>kidney>heart> urine>brain>blood in order). In 100 nm and 160 nm silver nanoparticles treatment groups, silver concentrations in the organs and body fluids were also increased in a dose-dependent manner. Blood biochemistry showed that GOT (glutamic oxaloacetic transaminase), ALP (alkaline phosphatase), TG (triglyceride), and BUN (blood urea nitrogen) values were significantly increased in the sera from high-dose group (5-10nm). Mutagenicity tests showed that Ag nanoparticles alone are not genotoxic, but genotoxic in combination of Fe++ or Cu++. These results suggest that silver nanoparticles, dependent on particle size and concenration, are able to be absorbed through the skin and may produce hepatotoxicity in vivo and genotoxicity in the presence of metal ions such as Fe++ or Cu++