Thermus caldophilus GK24로부터 내열성 alkaline phosphatase의 특성 = Properties of thermostable alkaline phosphatase from Thermus caldophilus GK24

다국어 초록 (Multilingual Abstract)

Thermostable alkaline phosphatase was found from Thermus caldophilus GK24, extreme thermophile. T caldophilus GK24 alkaline phosphatase was found to be inducible. When starved of inorganic phosphate, T caldophilus GK24 produces the enzyme alkaline phosphatase. The addition of inorganic phosphate to growth medium had a repressive effect on enzyme synthesis. T caldophilus GK24 was tested for the production of alkaline phosphatase by the addition of various concentrations of sodium glutamate, bactotryptone, glucose and yeast extract to basal salts. Sodium glutamate was found to be effective for the alkaline phosphatase induction. The enzyme was stable in the pH range of 7-12. The optimum temperature, pH of the enzyme were 75-85℃ and 11.0-11.5, respectively.

목차 (Table of Contents)

• 1. 서 론
• 2. 재료 및 방법
• 1) 균주 : Thermus caldophilus GK24
• 2) 배지 및 배양
• 3) 내열성 alkaline phosphatase의 활성 측정
• 4) 조효소액 제조
• 5) 비변성 겔 전기영동
• 6) pH의 안정성 측정
• 7) 최적 pH의 측정
• 8) 최적온도의 측정
• 3. 결과 및 고찰
• 1) Alkaline phosphatase 유도를 위한 질소원 탐색
• 2) 무기인산의 첨가 효과
• 3) pH의 안정성, 최적 pH 및 온도 측정