Hair growth promoting effect of plant complex extracts obtained from Chrysanthemum zawadskii, Mentha piperita and Glycyrrhiza glabra L. in C57BL/6 mice

다국어 초록 (Multilingual Abstract)

The conversion of testosterone to dihydrotestosterone play a crucial role in hair baldness. Recent approach showed specific inhibitors for 5a-reductase and androgen receptor promoted hair growth in male pattern alopecia. The purpose of this study was to investigate the in vivo effects of complex extracts from Chrysanthemum zawadskii, Mentha piperita and Glycyrrrhiza glabra (CMG) on hair growth growth by regulating androgen signaling proteins. We investigated the effects of antioxidant activity, Androgen signaling pathway proteins, inflammatory cytokine proteins analysis to determine the hair growth effects. Five-week-old male C57BL/6 mice were used. The mice were divided into the sevene groups: Normal control, Positive control, 100mg/kg CMG, 200mg/kg CMG, 400 mg/kg CMG, pansidil. Each group was orally administrated, once a day, for 14 days. We analyzed hair growth and histological changes. The results revealed that CMG treatment decreased the 5 α-reductase enzyme and androgen receptor protein level in mice. To investigate regulation of the inflammatory cytokine from CMG-treated mice, we conducted western blot analysis. Inflammatory cytokine proteins level in mice treated with CMG was changed in a dose dependent manner. Moreover, CMG treatment increased hair growth score and antioxidant activity in mice. Our in vivo results that CMG treatment showed the increase of hair follicles and hair growth. These results suggest that the CMG promotes hair growth by regulating the inhibition androgen signaling pathway proteins and activation of inflammatory cytokine and antioxidant activity.