Oxidative stress and chronic inflammatory processes are major risk factors for aging and age-related diseases. The present study investigated the antioxidant and anti-inflammatory effects of extracts derived from steam-exploded pine in UVB-exposed hum...
Oxidative stress and chronic inflammatory processes are major risk factors for aging and age-related diseases. The present study investigated the antioxidant and anti-inflammatory effects of extracts derived from steam-exploded pine in UVB-exposed human keratinocytes, HaCaT cells. The effects of extracts derived from steam-exploded pine on oxidative stress were examined in a UVB-induced aging model of human keratinocyte HaCaT cells. Extracts derived from steam-exploded pine protected cells from UVB-induced cytotoxicity (80 μg/mL) and reduced reactive oxygen species (ROS) production by UVB irradiation. Expression of genes associated with antioxidant effects, including superoxide dismutase 1 (SOD1) and catalase(CAT), increased in a dose-dependent manner. The expression of interleukin 6 (IL6), interleukin 6 (IL7), interleukin 8 (IL8), macrophage inflammatory protein-1β (MIP-1β), and monocyte chemoattractant Protein-1 (MCP-1) genes, which are associated with inflammation, also decreased in a dose-dependent manner. These results suggest that the extracts derived from steam-exploded pine may have an inhibitory effect on oxidative stress and inflammation.