지구성운동이 고혈압 동물의 지방조직 내 염증, 세포사멸 및 FOXO 발현에 미치는 효과 = The Effect of Endurance Exercise Training on Inflammatory Cytokines, Apoptosis, and FOXO Expression in Adipose Tissue of Hypertensive Rats

국문 초록 (Abstract)

본 연구의 목적은 중강도 지구성운동이 고혈압동물 지방조직에서 FOXO 단백의 변화와 염증성 사이토카인 및 세포사멸 조절에 미치는 영향을 규명하는데 있다. 실험동물은 정상혈압 통제군(WKY control, WKY), 고혈압 통제군(SHR control, SHR-C), 고혈압 운동군(SHR exercise, SHR-E)의 3집단으로 구성하고, 각 집단 간 9마리씩 무선배정 하였다. 지구성운동 집단은 16 m/min의 속도로 30~60분간, 5일/주, 총 16주 동안 트레드밀 운동을 실시하였다. 마지막 운동 24시간 후 모든 집단을 희생시키고 지방조직에서 p53, FOXO1, FOXO3a 단백질의 발현을 측정하였다. 그리고 염증성 사이토카인(IL-6, TNF-α, MCP-1)과 세포사멸(Bax, Caspase-9, Caspase-3) 발현은 RT-PCR을 이용하였다. 연구결과 16주간의 지구성 운동은 혈압을 유의하게 감소시켰다. 염증성 사이토카인(MCP-1과 TNF-α)과 세포사멸(Bax, Caspase-9, Caspase-3) 요인은 SHR-C에 비해 SHR-E에서 유의하게 감소되었다. 또한 p53 단백 발현은 SHR-C에 비해 SHR-E에서 유의하게 감소되었으나 FOXO1 발현은 유의하게 증가되었다. 이러한 결과들은 지구성운동이 고혈압의 지방조직에서 염증성 사이토카인과 세포사멸의 억제 및 FOXO 단백의 증가를 통해 혈압의 감소에 긍정적인 영향을 미칠 수 있다는 것을 시사한다.

다국어 초록 (Multilingual Abstract)

The purpose of this study was to investigate the expression of p53, FOXO1, FOXO3a, inflammatory cytokines and apoptosis in adipose tissue of endurance exercise trained rats. The Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were divided into 3 groups: WKY control (WKY, n=9), SHR control (SHR-C, n=9), SHR exercise (SHR-E, n=9), and submitted to treadmill exercise for 16 weeks (16 m/min, 5 days/week, 30-60 min/day). Every other weeks, systolic blood pressure were measured. After the last bout of exercise, all rats were sacrificed, then, p53, FOXO1, and FOXO3a were analyzed by western blotting. Also, inflammatory cytokines (IL-6, TNF-α, MCP-1) and apoptotic proteins(Bax, Caspase-9, and Caspase-3) were analyzed by RT-PCR. The expression of inflammatory cytokines (TNF-α, MCP-1) and apoptosis markers (Bax, Caspase-9, and Caspase-3) were significantly decreased in SHR-E compared to the SHR-C. Additionally, p53 was significantly decreased and FOXO1 was significantly increased by exercise in SHR rats. These findings sugges t that endurance exercise may decreases systolic blood pressure via enhancement of FOXO1 and suppression of inflammatory cytokines (TNF-α, MCP-1) and apoptosis factors (p53, Bax, Caspase-9, and Caspase-3) in adipose tissue of SHRs.

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