온도 구배가 있는 미세유체 장치를 이용한 극지 미생물의 형태 변화 분석

다국어 초록 (Multilingual Abstract)

We present microfluidic method to rapidly analyze the effect of temperature on the change of morphologies of Antarctic bacteria (Pseudoalteromonas sp., Shewanella vesiculosa, Shewanella sp., and Cellulophaga sp.). The microfluidic device is able to generate stable temperature gradient from 7 to 40℃ and dramatically reduce the number of experiments, experimental cost and labor, and amount of sample. Based on this approach, we found that specific bacteria transforming morphology into filament or elongated body strongly depends on cultivation temperature. Interestingly, we found that the morphologies of Pseudoalteromonas sp., Shewanella vesiculosa, Shewanella sp., and Cellulophaga sp. are elongated at below 25℃, above 20℃, above 15℃ and above 35℃, respectively. We envision the microfluidic device is a useful approach to analyze biological events with a high throughput manner.

목차 (Table of Contents)

• Abstract
• 1. INTRODUCTION
• 2. MATERIALS AND METHOD
• 3. RESULTS AND DISCUSSION
• 4. CONCLUSION
• REFERENCES

참고문헌 (Reference)

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