防風通聖散이 高脂肪飼料 食餌로 誘發된 肥滿생쥐에서 肥滿遺傳子 및 關聯因子에 미치는 影響 = (The) inhibitory effects of Bangpungtongseoungsan(防風通聖散) on the obese gene and obese inhibitory about obese-mouse induced by high fat diet

다국어 초록 (Multilingual Abstract)

In order to investigate the effects of Bangpungtongseoungsan(here in after referred to BPTS) on the obese gene and obese inhibitory, C57BL/6 mice were induced by high fat diet. C57BL/6 mice were divided into three groups(normal, high fat diet with control, high fat diet with BPTS extract) and fed for 13weeks.
Items of this experimental study are as follows.
Body weight change, diet intake change, final increse of body weight, the weight change of the adipocytes in body, the level change of ALT, AST, total cholesterol, LDL-Cholesterol, triglyceride, glucose, free fatty acid, total protein, albumin, A/G ratio, T-bilirubin and creatinine, the expression of β3AR and leptin gene in primary adipocytes, the expression of UCP-2, GLUT1, GLUT4, 5-HtP and LPL in primary adipocytes, the production change of TNF-α and leptin in primary adipocytes, the expression of leptin and β3AR in adipocytes tissue.
The following results have been obtained:
1. The change of body weight and the final increase of body weight were decreased significantly, and diet intake was a little decreased.
2. The amount of the adipocyte in body weight was decreased significantly.
3. The levels of ALT, total cholesterol, LDL-Cholesterol, glucose, free fatty acid were decreased significantly.
4. The levels of triglyceride, creatinine were decreased but did not show significance.
5. There were little change in levels of AST, total protein, albumin, A/G ratio, T-bilirubin.
6. In primary adipocytes, β3AR gene expression was increased significantly and leptin gene expression was decreased in 100 ㎍/㎖ density significantly.
7. In primary adipocytes, the expression of GLUT4, 5-HtP were decreased but did not show significance and there were little change in the expression of UCP-2, GLUT1, LPL.
8. In primary adipocytes, the amount of TNF-α was decreased in 100 ㎍/㎖ density significantly and the amount of leptin was decreased but did not show significance.
9. In adipocytes tissue, the expression of leptin was decreased and the expression of β3AR was increased.

목차 (Table of Contents)

• 目次 = ⅰ
• Ⅰ. 緖論 = 1
• Ⅱ. 實驗 = 3
• 1. 재료 = 3
• 1) 동물 및 사료 = 3
• 2) 약재 = 4
• 3) 시약 및 기기 = 5
• 2. 방법 = 6
• 1) 검액의 조제 = 6
• 2) 사료의 식이와 검액의 투여 = 6
• 3) 체중 측정 및 식이량 측정 = 7
• 4) 혈액학적 분석 = 7
• (1) 지방체조직, 채혈 및 혈청 분리 = 7
• (2) 간기능 검사 = 7
• (3) Cholesterol 함량 측정 = 7
• (4) 혈중 지질 함량 측정 = 7
• (5) Creatinine 함량 측정 = 8
• 5) 지방 체세포내 비만 세포 발현량 분석 = 8
• (1) In vitro = 8
• (2) In vivo = 8
• ① 지방세포의 분리 배양 및 ELISA 측정 = 8
• 가. 세포배양 = 8
• 나. TNF-α 및 leptin의 생산량 분석 = 9
• ② 지방세포내 leptin 과 β3AR 발현량 조사 = 9
• 가. 지방세포의 분리와 RNA 추출 = 9
• 나. 역전사-중합효소 연쇄반응 (RT-PCR) = 10
• 다. cDNA PCR = 10
• 6) 통계처리 = 12
• Ⅲ. 成績 = 13
• 1. 체중변화에 미치는 영향 = 13
• 2. 최종 체중 증가량에 미치는 영향 = 14
• 3. 사료 섭취량의 변화 = 15
• 4. 체중내 adipocyte 중량에 미치는 영향 = 16
• 5. 혈청에 미치는 영향 = 17
• 6. Primary adipose cell에서의 유전자 발현에 미치는 영향 = 32
• 1) β3AR 발현에 미치는 영향 = 32
• 2) Leptin 발현에 미치는 영향 = 33
• 3) UCP-2 발현에 미치는 영향 = 34
• 4) GLUT1 발현에 미치는 영향 = 35
• 5) GLUT4 발현에 미치는 영향 = 36
• 6) LPL 발현에 미치는 영향 = 37
• 7) 5-HtP 발현에 미치는 영향 = 38
• 8) TNF-α의 생성량에 미치는 영향 = 39
• 9) Leptin의 생성량에 미치는 영향 = 40
• 7. Adipocytes tissue에서의 유전자 발현에 미치는 영향 = 41
• 1) Leptin 발현에 미치는 영향 = 41
• 2) β3AR 발현에 미치는 영향 = 42
• Ⅳ. 考察 = 43
• Ⅴ. 結論 = 55
• 參考文獻 = 57
• Abstract = 63