Effects of 5,7-dihroxytryptamine administered supraspinally or spinally on the blood glucose level in D-glucose-fed and immobilization stress models

다국어 초록 (Multilingual Abstract)

5,7-Dihydroxytryptamine (5,7-DHT) is a neurotoxin which causes the depletion of serotonin. Moreover, the serotonergic system is the regulator of the blood glucose level. However, the role of centrally located serotonergic system in blood glucose regulation after D-glucose feed and immobilization (IMO) stress was not clearly characterized yet. Thus the present study was designed to examine the effect of 5,7-DHT administered intracerebroventricularly (i.c.v.) or intrathecally (i.t.) on the blood glucose level in D-glucose-fed and immobilization stress models. Mice were pretreated once i.c.v. or i.t. with 5,7-DHT (from 10 to 40 μg) for 3 days and D-glucose (2 g/kg) was fed orally. The blood glucose level was measured at 0, 30, 60 and 120 min after Dglucose feeding and immobilization stress initiation. We found that i.c.v. or i.t. pretreatment with 5,7-DHT attenuated the blood glucose level in both animal models. D-glucose feeding causes an increase in plasma insulin level, whereas the plasma corticosterone level was downregulated in the D-glucose-fed model. The i.c.v. or i.t. pretreatment with 5,7-DHT alone slightly increased the plasma corticosterone level. In addition, the i.c.v. or i.t. pretreatment with 5,7-DHT caused a reversal of the downregulation of plasma corticosterone level induced by D-glucose feeding, whereas immobilization stress causes an increase in plasma corticosterone and insulin levels. The i.c.v or i.t. pretreatment with 5,7-DHT attenuated the immobilization stress-induced plasma corticosterone and plasma insulin levels. Our results suggest that supraspinal and spinal depletion of serotonin appears to be responsible for the downregulation of blood glucose level in both D-glucose-fed and immobilization stress models.

참고문헌 (Reference)

1 Fagerholm V, "alpha2-adrenoceptor regulation of blood glucose homeostasis" 108 : 365-370, 2011

2 Sim YB, "The modulatory role of alpha-melanocyte stimulating hormone administered spinally in the regulation of blood glucose level in d-glucose-fed and restraint stress mouse models" 48 : 207-212, 2014

3 Hornung JP., "The human raphe nuclei and the serotonergic system" 26 : 331-343, 2003

4 Sugimoto Y, "The effects of the serotonin1A receptor agonist buspirone on the blood glucose and pancreatic hormones in rats" 60 : 145-148, 1992

5 Lechin F, "The effect of serotonin (5-HT) on insulin secretion" 25 : 339-346, 1975

6 Finnegan KT, "The amine-depleting effects of 5,7-dihydroxytryptamine (5,7-DHT) in C57BL/6 mice do not increase with age" 496 : 251-256, 1989

7 Jørgen Jensen, "The Role of Skeletal Muscle Glycogen Breakdown for Regulation of Insulin Sensitivity by Exercise" Frontiers Media SA 2 : 2011

8 Nonogaki K, "Stress, acute hyperglycemia, and hyperlipidemia role of the autonomic nervous system and cytokines" 8 : 192-197, 1997

9 Lin MT, "Stimulation of 5-hydroxytryptamine nerve cells in dorsal and median raphe nuclei elevates blood glucose in rats" 417 : 441-445, 1991

10 Chi TC, "Serotonin enhances beta-endorphin secretion to lower plasma glucose in streptozotocin-induced diabetic rats" 80 : 1832-1838, 2007

11 Glick G, "Role of the autonomic nervous system in the circulatory response to acutely induced anemia in unanesthetized dogs" 43 : 2112-2124, 1964

12 Clowes Jr GH., "Pulmonary abnormalities in sepsis" 54 : 993-1013, 1974

13 Chaouloff F., "Physiopharmacological interactions between stress hormones and central serotonergic systems" 18 : 1-32, 1993

14 HaleyTJ., "Pharmacological effects produced by intracerebral administration of drugs of unrelated structure to conscious mice" 110 : 239-244, 1957

15 Aoki K, "Mosapride citrate, a 5-HT(4) receptor agonist, increased the plasma active and total glucagon-like peptide-1 levels in non-diabetic men" 60 : 493-499, 2013

16 Uresin Y, "Losartan may prevent the elevation of plasma glucose, corticosterone and catecholamine levels induced by chronic stress" 5 : 93-96, 2004

17 Suh HW, "Involvement of dynorphin in immobilization stress-induced antinociception in the mouse" 10 : 407-413, 2000

18 강유정, "Involvement of a2-adrenergic receptor in the regulation of the blood glucose level induced by immobilization stress" 대한약학회 38 (38): 921-929, 2015

19 Hylden JL, "Intrathecal substance P elicits a caudally-directed biting and scratching behavior in mice" 217 : 212-215, 1981

20 김채하, "Intracerebroventricular Kainic Acid-Induced Damage Affects Blood Glucose Level in d-glucose-fed Mouse Model" 한국뇌신경과학회 24 (24): 24-30, 2015

21 Baum D, "Inhibition of insulin release in infants undergoing deep hypothermic cardiovascular surgery" 279 : 1309-1314, 1968

22 Peschke E, "Influence of melatonin and serotonin on glucose-stimulated insulin release from perifused rat pancreatic islets in vitro" 23 : 156-163, 1997

23 Heimes K, "Impact of the 5-HT3receptor channel system for insulin secretion and interaction of ginger extracts" 624 : 58-65, 2009

24 Carvalho F, "Hyperglycemia induced by acute central fluoxetine administration: role of the central CRH system and 5-HT3 receptors" 38 : 98-105, 2004

25 Kudielka BM, "Human models in acute and chronic stress: assessing determinants of individual hypothalamus-pituitary-adrenal axis activity and reactivity" 13 : 1-14, 2010

26 Chaouloff F, "Ganglionic transmission is a prerequisite for the adrenaline-releasing and hyperglycemic effects of 8-OH-DPAT" 185 : 11-18, 1990

27 Chaouloff F, "Effects of the 5-HT1C/5-5-HT2 receptor agonists DOI and alpha-methyl-5-HT on plasma glucose and insulin levels in the rat" 187 : 435-443, 1990

28 Pontiroli AE, "Effects of serotonin, of its biosynthetic precursors and of the anti-serotonin agent metergoline on the release of glucagon and insulin from rat pancreas" 10 : 200-203, 1978

29 Anderson RE, "Effects of glucose and PaO2 modulation on cortical intracellular acidosis, NADH redox state, and infarction in the ischemic penumbra" 30 : 160-170, 1999

30 Hall FS, "Effects of 5,7-dihydroxytryptamine depletion of tissue serotonin levels on extracellular serotonin in the striatum assessed with in vivo microdialysis: relationship to behavior" 33 : 16-25, 1999

31 Gadek-Michalska A, "Effect of prior stress on interleukin-1beta and HPA axis responses to acute stress" 63 : 1393-1403, 2011

32 Kolta MG, "Effect of hyperglycemia on biogenic amines, beta-endorphin and insulin of the rat brain" 16 (16): 16-20, 1984

33 서홍원, "Effect of Sulfonylureas Administered Centrally on the Blood Glucose Level in Immobilization Stress Model" 대한약리학회 19 (19): 197-202, 2015

34 Adeghate E, "Distribution of serotonin and its effect on insulin and glucagon secretion in normal and diabetic pancreatic tissues in rat" 20 : 315-322, 1999

35 Monti JM, "Depletion of brain serotonin by 5,7-DHT: effects on the 8-OH-DPAT-induced changes of sleep and waking in the rat" 115 : 273-277, 1994

36 Luo S, "Chronic ventromedial hypothalamic infusion of norepinephrine and serotonin promotes insulin resistance and glucose intolerance" 70 : 460-465, 1999

37 Yettefti K, "Characteristics of glycemiasensitive neurons in the nucleus tractus solitarii: possible involvement in nutritional regulation" 61 : 93-100, 1997

38 Jansen AS, "Central command neurons of the sympathetic nervous system: basis of the fight-or-flight response" 270 : 644-646, 1995

39 Tork I., "Anatomy of the serotonergic system" 600 : 9-34, 1990

40 Shimizu H., "Alteration in hypothalamic monoamine metabolism of freely moving diabetic rat" 131 : 225-227, 1991

41 Lopez JF, "A.E. Bennett Research Award. Regulation of serotonin1A, glucocorticoid, and mineralocorticoid receptor in rat and human hippocampus:implications for the neurobiology of depression" 43 : 547-573, 1998

42 Baum D, "A mechanism for regulation of insulin release in hypoxia" 222 : 695-699, 1972