간접 혈구응집반응(IHA)을 이용한 간흡충증의 혈청학적 진단에 관한 연구 = Evaluation of Indirect Hemagglutination Test with Adult Worm Antigen for the Serodiagnosis of Clonorchiasis

다국어 초록 (Multilingual Abstract)

Detection of infections with Clonorchis sinensis has been accomplished through various serodiagnostic procedure including complement fixation test, gel diffusion test, indirect fluorescent antibody test and enzyme linked immunosorbent assay etc. In 1960, Pacheco, et al. reported that indirect hemagglutination test showed relatively more sensitive reaction than CFT in experimentally infected animal and 3 human cases. However, more detailed studies on the use of IHA in serodiagnosis of clonorchiasis were not accomplished. Evaluation of an indirect hemagglutination test was made in 100 egg positive cases in comparison with 51 parasite infected cases other than C. sinensis and 58 parasite free control. Kagan's modification of Boyden technique was employed using ether treated borate buffer extract adult worm antigen with 0.5㎎/ml concentration by Chaffee's method.
The results obtained were as follows:
1. Ether treated borate buffer extract adult worm antigen was more sensitive than that of veronal buffered salt extract.
2. Sensitivity of IHA test was 84.0%) among 100 Clonorchs egg positive cases when cut-off titer of 1:16 was applied. Only 1 control sera out of 58 cases(98.3%) specificity) showed positive reaction.
3. Sensitivity was only 80.0% in light infection, 87.1% in moderate infection. However, only 1 case showed false negative reaction among 14 cases in heavy and very heavy infection category.
4. ELISA was more sensitive than IHA test, showing 90.0% sensitivity and 98.3% specificity.
5. Only 2 cases infected with Paragonimus westermani showed positive cross reaction in either IHA test or ELISA.
6. A positive correlation was found between results of ELISA and IHA test of patients with clonorchiasis. And the results obtained in both techniques were same in 86% of positive sera and 100% of negative sera. In conclusion, it is suggested that in addition to ELISA, IHA is a relatively sensitive and reliable test for the sere-diagnosis and sero-epidemiological survey of C. sinensis infection.