Effect of indoleacetic acid and kinetin on glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in tobacco tissue cultures에 미치는 영향 = 담배조직 배양에서 indoleacetic acid와 kinetin이 glucose-6-phosphate dehydrogenase와 6-phosphogluconate dehydrogena

국문 초록 (Abstract)

담배조직배양의 배지에 indole-3-acetic acid(IAA)와 kinetin을 정상 농도보다 아주 높거나 낮게 첨가한 후 glucose-6-phosphate dehydrogenase 와 6-phosphogluconate dehydrogenase의 활성도와 isoenzyme pattern에 미치는 영향을 조사하였다. 정상 농도보다 훨씬 낮은 IAA농도(10^-8M)와 높은 kinetin 농도(10^-5M)에서는 두 효소의 활성이 대부분의 배양 기간동안 대조군보다 약간 높게 나타났다. 한편 정상 농도보다 아주 높은 IAA 농도(10^-3M)와 낮은 kinetin 농도 (10^-9M)에서는 대부분의 배양 기간동안 대조군보다 낮은 활성을 유지하였다. IAA와 kinetin의 농도 변화에 따른 두 효소의 이러한 활성변화는 전기영동을 통하여 살펴본 두 효소의 isoenzyme pattern 변화에서도 그대로 나타났다. 즉 담배조직배양에서 HMP shunt 조절효소들에 대한 두 hormone의 영향은 서로 상반됨을 알 수 있었다.

다국어 초록 (Multilingual Abstract)

The effect of hormone stress on the activities of glucose-6-phosphate deydrogenase and 6-phosphogluconate dehydrogenase in culture of Nicotiana tabacum was examined. In the extremely low IAA concentration range (10^-8M) and high kinetin level (10^-5M), the activities of HMP shunt enzymes were enhanced during most culture period as compared to the growth promoting normal IAA and kinetin concentrations. In high toxic IAA concentration range (10^-3M) on the other hand, the level of both enzymes was reduced significantly throughout culture. The slight decrease in enzyme activity also occurred when the tissue was grown on very low kinetin level (10^-9M). The results obtained in the activity profile experiments were also reflected by isoenzyme pattern studies performed at different hormone concentrations.