肝臟損傷이 肝의 Superoxide Dismutase에 미치는 影響 = Influence of Liver Injury on Hepatic Superoxide Dismutase

국문 초록 (Abstract)

간장손상시 간의 superoxide dismutase의 변화를 알아보기위하여 흰쥐에게 CCl_4를 투여하거나 또는 총담관을 결찰하고 시간경과에 따른 간장의 superoxide dismutase 변동을 관찰하여 다음과 같은 결과를 얻었다.
정상흰쥐 간세포에서의 superoxide dismutase 분포는, 세포질에는 세포 총 superoxide dismutase의 95.2%. mitochandria에는 4.8%가 분포되어 있었다. CCl_4단회 투여시에는, 세포질 superoxide dismutase는 투여후 6시간네 감소되기 시작하여 24시간에 최저치를보이다가 이후 증가하여 72시간에는 대조군의 약 82%까지 회복되는 경향이었고, mitochondrial superoxide dismutase는 투여후 12시간에 감소하기 시작하여 24시간에 최저치를 나타내고 이후 증가하여 72시간에는 대조군의 약 79%까지 회복되는 경향이었다.
CCl_4간격투여시에는 세포질, mitochondrial superoxide dismutase 모두 투여후 9일까지 감소되어 회복되지 않았다.
CCl_4 간격투여시에는 세포질, mitochondrial superoxide dismutase 모두 투여후 6일까지는 감소되어 있었으나 9일에는 다소 회복되는 경향이었다. 그리고 총담관 결찰시에는 의의있는 변동을 볼 수가 없었다. 이상의 결과로 보아 CCl_4에 의한 간손상시에는 간세포내 superoxide dismutase가 감소하며 세포질의 superoxide dismutase 감소가 mit-ochondrial superoxide dismutase의 감소보다 빨리 일어나나, 담관결찰시에는 이 효소의 변동이 없는 것을 알 수 있었다.

다국어 초록 (Multilingual Abstract)

To study the influence of liver injury on hepatic superoxide dismutase in rats, superoxide dismutase was determined in rat liver after either CCl_4 injection or common bile duct ligation.
In normal rat liver cells, 95.2% of total cellular superoxide dismutase was distributed in cytosol and 4.8% was in mitochondria, respectively.
In single injection of CCl_4, cytocolic superoxide dismutase was decreased at 6 hours after injection and revealed minimum value at 24 hours, thereafter increased to 82% of the control level at 72 hours. Mitochondrial superoxide dismutase was decreased at 12 hours after injection and revealed minimum value at 24 hours while increasing to 79% of the control level at 72 hours after injection.
In 2 day-interval injection of CCl_4, both cytosolic and mitochondrial superoxide dismutase showed decreased levels until 9 days after the first injection and did not recover.
In 3 dua-interval injection of CCl_4, both cytosolic and mitochondrial superoxide dimutase showed decreased levels until 6 days after the first injection and recovered to 75% and 64% of the control at 9 days, respectively.
No significant changes were noted after common bile duct ligation.
All of these findings are compatible with the views that cellular superoxide dismutase decreases in CCl_4-induced liver injury and decrease of cytosolic superoxide dismutase precedes that of mitochondrial superoxide dismutase. But superoxide dismutase did not change after the ligation of common bile duct.