RISS 검색 - 국내학술지논문 상세보기

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다국어 초록 (Multilingual Abstract)

In this experiment, it was attempted to observe the possible effect of Korean ginseng saponin on the growth and one of the most important enzyme in TCA cycle, succinate dehydrogenase (E.C. 1. 3.99. 1. ), of E. coli w3110 to make a contribution to revealing partially the physiological action of ginseng saponin. The following results were obtained: 1) When the concentrations of ginseng saponin in the assay mixture were between 1×10^(-1) % and 6×10^(-1)%, E. coli soluble SDH was highly activated; the maximum activity of E. coli soluble SDH was shown in the presence of 4×10^(-1) ginseng saponin and its activity was inhibited below 1×10^(-2)%. The ㎞ value for succinate of E. coli soluble SDH was 5.05×10^(-3)M. 2) At 4×10^(-1)% saponin solution, E. coli membrane bound SDH was inhibited significantly but concentrations above 2×10^(-1)% saponin solution were innocuous for the enzyme. The km value for succinate of E, coli membrane bound SDH was 2.58×10^(-3)M. 3) When the E. coli were grown in the media containing 1×10^(-1)% ginseng saponin, there was increase in lag phase of E. coli. However, the growth curve of E. coli was similar to that of control when the concentrations of ginseng saponin was between 1×10^(-2)% and 1×10^(-4)%. 4) When the E. coli were grown in the media containing 1×10^(-1)% ginseng saponin, the activity of soluble SDH was increased 26% in compare with that of control whereas the activity of soluble SDH seemed to be degreased in E. coli grown in the media containing 1×10^(-2) and 1×10^(-3)%. 5) After preincubation at various temperature, E. coli soluble SDH was highly activated when the concentration of ginseng saponin was 4×10^(-1)%. The optimum temperature of E. coli soluble SDH was at 2℃ under condition described. 6) After storage with and without ginseng saponins at 2℃ for 9,21, and 27 hours, the activities of soluble SDH were almost similar.