2 - Acetylaminofluorene 의 투여량에 따른 쥐간의 Monooxygenase 에 관한 쇠기름 및 콩기름의 영향 = Effects of Beef Tallow and Soybean Oil on Hepatic Monooxygenases in Rats Treated with Different Levels of 2 - acetylaminofluorene

다국어 초록 (Multilingual Abstract)

Weanling Sprague Dawley male rats were fed the diet containing 15% beef tallow and soybean oil for 8 weeks. At 9th week, 2-AAF was injected everyday for 3 days by intraperitoneally at the levels of 25 ㎎ and 100 ㎎/㎏ body weight and sacrificed next day. Cytochrome P-450 and cytochrome b_5 contents, NADPH-cytochrome P-450 reductase activity, lipid peroxide values were determined from isolated microsome. Cytochrome P-450 contents and lipid peroxide values were significantly higher in soybean oil group than beef tallow group by 25 ㎎ 2-AAF injection, and cytochrome P-450 contents decreased but cytochrome b_5 contents increased in soybean oil group by 100 ㎎ 2-AAF injection. NADPH-cytochrome P-450 reductase activities were not significantly different between dietary groups, but were elevated by 2-AAF injection and positively correlated to the levels of 2-AAF in each dietary group. These results suggest that soybean oil is more susceptible to the damage as well as induction of cytochrome P-450 by 2-AAF, and that lipid peroxide is not positively correlated to the cytochrome P-450 contents.