넙치, Paralichthys olivaceus의 미세플라스틱 급성 노출에 따른 혈액성상 및 혈장성분의 변화 = Changes in hematological parameters and plasma components of olive flounder, Paralichthys olivaceus exposed to acute microplastics

다국어 초록 (Multilingual Abstract)

Juvenile olive flounder, Paralichthys olivaceus (mean weight 66.7±7.1 g; mean length 19.2±0.9 cm) in a bio-floc environment were exposed to microplastic (PE: polyethylene, size 40-48 ㎛) at 0, 4, 20, 100, 500 and 2,500 mg L^-1 for 96 hours. No P. olivaceus deaths were observed following microplastic exposure. In the plasma components, calcium was significantly decreased whereas there was no significant change with magnesium following microplastic exposure. Glucose was significantly decreased with over 100mg L^-1 at 48 hours and 20mg L^-1 at 96 hours. Cholesterol was significantly decreased with over 20mg L^-1 after 48 hours, whereas there was no significant change in the total protein content. In enzymatic plasma components, the AST(Aspartate aminotransferase) was significant decreased by microplastic exposure. The results of this study indicate that acute exposure to microplastic induces blood physiological changes in P. olivaceus.

국문 초록 (Abstract)

본 실험에서 미세플라스틱의 노출은 넙치의 혈액학적 성상인 hemoglobin 및 hematocrit 수치의 유의적인 감소를 유발하였다. 또한 미세플라스틱의 노출은 넙치 혈장 무기성분인 calcium, 유기성분인 glucose 및 cholesterol, 효소성분인 AST의 유의적인 변화를 나타냈다. 본 연구에서 바이오플락 환경에서 미세플라스틱의 노출이 어류의 혈액생리에 영향을 주며 독성으로 작용하는 것을 확인할 수 있었다. 향후, 일반 해수와 바이오플락 환경으로 각각 양성한 넙치를 이용하여 사육환경의 변화에 의한 미세플라스틱 노출영향의 차이에 대해서도 추가적인 연구가 이루어져야 할 것이다. 본 연구는 다양한 어종에서 종별 미세플라스틱의 농도 연구에서 미세플라스틱 노출에 따른 종별 독성영향비교평가를 위한 기본 자료로 활용될 수 있을 것이다. 하지만, 바이오플락 환경에서 미세플라스틱의 응집은 미세플라스틱의 독성에 영향을 미치는 중요한 요소이기 때문에(Choi et al. 2020), 향후 연구에서 철저한 모니터링과 함께 다양한 미세플라스틱 독성에 미치는 요소에 대한 추가연구가 필요할 것이다.

참고문헌 (Reference)

1 최병규, "정수처리장 내의 미세플라스틱의 유입 및 처리기술 현황에 관한 고찰" 대한상하수도학회 34 (34): 357-371, 2020

2 김광민, "암모니아 급성노출에 따른 대왕범바리 (Epinephelus fuscoguttatus ♀× E. lanceolatus ♂)의 혈액성상 및 혈장성분의 변화" 한국환경생물학회 38 (38): 40-46, 2020

3 김준환, "바이오플락 양식기술 적용 넙치, Paralichthys olivaceus 수용밀도에 따른 수질변화 및 혈액학적 성상 변화" 한국환경생물학회 37 (37): 155-163, 2019

4 조영록, "바이오플락 기술을 활용한 순환침전시스템에 따른 넙치 (Paralichthys olivaceus)의 양성" 한국환경생물학회 37 (37): 129-135, 2019

5 한선기, "미세플라스틱의 개념과 해양에서의 발생, 이동, 생물영향 및 관리대책" 한국환경보건학회 46 (46): 610-626, 2020

6 조재황, "대왕범바리 (Epinephelus fuscoguttatus ♀×E. lanceolatus ♂)의 아질산 급성노출에 따른 내성한계: 혈액성상 및 혈장성분의 변화" 한국환경생물학회 38 (38): 93-100, 2020

7 전규호, "김치 유산균 배양물의 부상용 배합사료내 첨가시 넙치P(aralichthys olivaceus) 치어의 성장, 체조성 및 면역성 반응에 미치는 효과" 한국수산과학회 46 (46): 552-558, 2013

8 Karami A, "Virgin microplastics cause toxicity and modulate the impacts of phenanthrene on biomarker responses in African catfish(Clarias gariepinus)" 151 : 58-70, 2016

9 Yong CQY, "Toxicity of microplastics and nanoplastics in mammalian systems" 17 : 1509-, 2020

10 Pannetier P, "Toxicity assessment of pollutants sorbed on environmental microplastics collected on beaches : Part II-adverse effects on Japanese medaka early life stages" 248 : 1098-1107, 2019

11 Kim JH, "Toxic effects on bioaccumulation, hematological parameters, oxidative stress, immune responses and neurotoxicity in fish exposed to microplastics:A review" 413 : 125423-, 2021

12 Kim JH, "Toxic effects on bioaccumulation and hematological parameters of juvenile rockfish Sebastes schlegelii exposed to dietary lead(Pb)and ascorbic acid" 176 : 131-140, 2017

13 Ter Halle A, "To what extent are microplastics from the open ocean weathered?" 227 : 167-174, 2017

14 Su L, "The occurrence of microplastic in specific organs in commercially caught fishes from coast and estuary area of east China" 365 : 716-724, 2019

15 Azim ME, "The biofloc technology (BFT) in indoor tanks: water quality, biofloc composition, and growth and welfare of Nile tilapia (Oreochromis niloticus)" 283 : 29-35, 2008

16 Krishnapriya K, "Sublethal concentration of bisphenol A induces hematological and biochemical responses in an Indian major carp Labeo rohita" 17 : 306-313, 2017

17 Svoboda M, "Stress in fishes. A review" 37 : 169-191, 2001

18 Wen B, "Single and combined effects of microplastics and cadmium on the cadmium accumulation, antioxidant defence and innate immunity of the discus fish (Symphysodon aequifasciatus)" 243 : 462-471, 2018

19 강주찬, "Pentachlorophenol 노출에 따른 넙치, Paralichthys olivaceus의 혈액성분의 변화" 한국어병학회 23 (23): 189-198, 2010

20 Siddeswaran S, "Non-Steroidal Anti-Inflammatory Drugs in Water: Emerging Contaminants and Ecological Impact" Springer 191-215, 2020

21 Iheanacho SC, "Neurotoxicity, oxidative stress biomarkers and haematological responses in African catfish (Clarias gariepinus) exposed to polyvinyl chloride microparticles" 232 : 108741-, 2020

22 Burgos-Aceves MA, "Multidisciplinary haematology as prognostic device in environmental and xenobiotic stress-induced response in fish" 670 : 1170-1183, 2019

23 Chen Q, "Microplastics lead to hyperactive swimming behaviour in adult zebrafish" 224 : 105521-, 2020

24 Roda JFB, "Microplastics and copper effects on the neotropical teleost Prochilodus lineatus: Is there any interaction?" 242 : 110659-, 2020

25 Lei L, "Microplastic particles cause intestinal damage and other adverse effects in zebrafish Danio rerio and nematode Caenorhabditis elegans" 619 : 1-8, 2018

26 Bijvelds MJ, "Magnesium transport in freshwater teleosts" 201 : 1981-1990, 1998

27 Villanueva J, "Intestinal alkaline phosphatase of the fish Cyprinus carpio : regional distribution and membrane association" 279 : 347-355, 1997

28 Browne MA, "Ingested microscopic plastic translocates to the circulatory system of the mussel, Mytilus edulis (L.)" 42 : 5026-5031, 2008

29 Suvetha L, "Influence of cypermethrin toxicity on ionic regulation and gill Na+/K+-ATPase activity of a freshwater teleost fish Cyprinus carpio" 29 : 44-49, 2010

30 Fırat Ö, "Individual and combined effects of heavy metals on serum biochemistry of Nile tilapia Oreochromis niloticus" 58 : 151-157, 2010

31 Javed M, "Impact of heavy metal toxicity on hematology and glycogen status of fish: a review" 85 : 889-900, 2015

32 Corcoran PL, "Hidden plastics of Lake Ontario, Canada and their potential preservation in the sediment record" 204 : 17-25, 2015

33 Srivastava B, "Haematological and Serum Biomarker Responses in Heteropneustes fossilis Exposed to Bisphenol A" 19 : 1577-1584, 2020

34 Ramaswamy M, "Glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) enzyme activities in different tissues of Sarotherodon mossambicus (Peters) exposed to a carbamate pesticide, carbaryl" 55 : 1217-1221, 1999

35 Banaee M, "Evaluation of single and combined effects of cadmium and micro-plastic particles on biochemical and immunological parameters of common carp(Cyprinus carpio)" 236 : 124335-, 2019

36 Banaee M, "Effects of microplastic exposure on the blood biochemical parameters in the pond turtle (Emys orbicularis)" 28 : 9221-9234, 2020

37 Haghi BN, "Effects of micro-plastic particles on paraquat toxicity to common carp(Cyprinus carpio) : biochemical changes" 14 : 521-530, 2017

38 Pratap HB, "Effect of waterborne and dietary cadmium on plasma ions of the teleost Oreochromis mossambicus in relation to water calcium levels" 18 : 568-575, 1989

39 Baby J, "Effect of curacron toxicity on aminotransferases(ALT and AST)in the serum of the fish Cyprinus carpio" 5 : 207-211, 2011

40 Iheanacho SC, "Dietary exposure to polyvinyl chloride microparticles induced oxidative stress and hepatic damage in Clarias gariepinus (Burchell, 1822)" 27 : 1-15, 2020

41 Dreolin N, "Development and validation of a LC-MS/MS method for the analysis of bisphenol a in polyethylene terephthalate" 274 : 246-253, 2019

42 Flik G, "Calcium transport in fish gills and intestine" 184 : 17-29, 1993

43 Suzuki N, "Both mercury and cadmium directly influence calcium homeostasis resulting from the suppression of scale bone cells : the scale is a good model for the evaluation of heavy metals in bone metabolism" 22 : 439-446, 2004

44 Suzuki N, "Bisphenol A influences the plasma calcium level and inhibits calcitonin secretion in goldfish" 20 : 745-748, 2003

45 Lallès JP, "Biology, environmental and nutritional modulation of skin mucus alkaline phosphatase in fish: A review" 89 : 179-186, 2019

46 Crab R, "Biofloc technology in aquaculture : beneficial effects and future challenges" 356 : 351-356, 2012

47 Kim JH, "Bio-floc technology application in flatfish Paralichthys olivaceus culture: Effects on water quality, growth, hematological parameters, and immune responses" 495 : 703-709, 2018

48 Fazio F, "Automatic analysis to assess haematological parameters in farmed gilthead sea bream (Sparus aurata Linnaeus, 1758)" 45 : 63-73, 2012

49 Hamed M, "Assessment the effect of exposure to microplastics in Nile Tilapia (Oreochromis niloticus) early juvenile: I. blood biomarkers" 228 : 345-350, 2019

50 Montuori P, "Assessing human exposure to phthalic acid and phthalate esters from mineral water stored in polyethylene terephthalate and glass bottles" 25 : 511-518, 2008

51 Kumar R, "Arsenic induced hematological and biochemical responses in nutritionally important catfish Clarias batrachus (L.)" 3 : 148-152, 2016

52 Annune PA, "Acute toxicity of cadmium to juveniles of Clarias gariepinus (Teugels) and Oreochromis niloticus (Trewavas)" 29 : 1357-1365, 1994

53 Mak CW, "Acute toxic effects of polyethylene microplastic on adult zebrafish" 182 : 109442-, 2019

54 Molina R, "Acid and alkaline phosphatase activities and pathological changes induced in Tilapia fish (Oreochromis sp.) exposed subchronically to microcystins from toxic cyanobacterial blooms under laboratory conditions" 46 : 725-735, 2005

55 Yu P, "Accumulation of polystyrene microplastics in juvenile Eriocheir sinensis and oxidative stress effects in the liver" 200 : 28-36, 2018

56 Pottinger TG, "A comparison of plasma glucose and plasma cortisol as selection markers for high and low stress-responsiveness in female rainbow trout" 175 : 351-363, 1999