In order to investigate annumor and immune response effect by Hyangsapyungwisan after Sarcoma-
180 cells and methotrexate were treared each other. the extract of Hyangsapyungwisan was orally
administered to ICR mice for 14 days.
To evaluate the eff...
In order to investigate annumor and immune response effect by Hyangsapyungwisan after Sarcoma-
180 cells and methotrexate were treared each other. the extract of Hyangsapyungwisan was orally
administered to ICR mice for 14 days.
To evaluate the effects of the Hyangsapyungwisan, 50% inhibition concentration(IC_50), mean survival days, tumor weight for antitumor effects, hemagglutinin titer, hemolysin titer, rosette forming cells,
natural killer cell and productivity of interleukin-2 for immune responses measured in ICR mice.
The results were summarized as follows:
1. Mean survival time in Hyangsapyungwisan-treated group was slightly prolonged, as compared with control group(13.46%).
2. On the MTT assay, cell viability was significantly inhibited by 5ug/well, 2.5ug/well, l.25ug/well.
and 0.625ug/well of Hyangsapyung-wism concentration inhibited cell viability significantly. IC_5o for cell viability was 11.59ug/well.
3. Tumor weight in Hyangsapyungwisan treated group was depressed, as compared with the control group(P<0.05).
4. Hemagglutinin titer in Hyangsapyungwisan-treated group was slightly increased with no
significance, as compared with the control group.
5. Hemolysin titer in Hyangsapyungwisan-treated group was silightly increased, as compared with the control group(P<0.05).
6. Rosette forming cells in Hyangsapyungwisan-treated group was silightly increased, as compared with the control group(P<0.05).
7. Naural killer cell activity in Hyangsapyungwisan-treated group was significantly increased(P<0.05).
8. Production of interleukin-2 was significantly increased(P<0.05).
According to the above results, Hyangsapygwisan had prominent antitumor, and enhance both cellular and humoral immunity in mice.