왕대의 첨가수준이 반추위 in vitro 발효성상과 메탄 발생량에 미치는 영향 = Effects of Additional Levels of Phyllostachys bambusoides on Ruminal Fermentation Characteristics and Methane Emission in in vitro

다국어 초록 (Multilingual Abstract)

The current study was to evaluate the antioxidant activity of Phyllostachys bambusoides (PHB) as a feed additives and investigate whether its antioxidant activity could be helpful for increasing rumen fermentation characteristics and methane reduction. The antioxidant activity results showed that total polyphenols and flavonoids contents were 43.54 ± 8.68 mg CE/g and 17.13 ± 0.45 mg QE/g, respectively, and the IC₅₀ values for 1,1-diphenyl-2-prcrylhydrazyl (DPPH) and 2,2′-azino-bis (3- ethylbenzthiazoline-6- sulphonic acid) (ABTS) radical scavenging activity were 163.13 ± 19.25 μg/mL and 97.07 ± 4.46 μg/mL, respectively. Two heads of cannulated Hanwoo (450 ± 30 kg), consuming timothy hay and a commercial concentrate (60:40, w/w) twice daily (at 09:00 and 17:30) at 2% of body weight, with free access to water and a mineral block, were used as rumen fluid donors. An in vitro incubation experiment was performed after 6, 12, 24, 48, and 72 hr with PHB added at concentration of 2, 4, and 6% of timothy hay basis. Total gas emission decreased as the amount of PHB addition increased at 6 and 24 hr of incubation. However, PHB addition did not affect total volatile fatty acid production, and methane and carbon dioxide emission also decreased as the amount of addition increased at 48 hr of incubation. Therefore, PHB was expected to be used as methane reducing additives in the ruminants.

참고문헌 (Reference)

1 이민자, "한국산 왕대, 솜대, 맹종죽, 조릿대 및 오죽의 항산화 효과" 한국식품과학회 35 (35): 1226-1232, 2003

2 김다송, "추출용매에 따른 국내산 대나무 잎의 폴리페놀 함량 및 항산화 활성" 공학기술연구원 11 (11): 7-13, 2018

3 주인옥, "추출방법에 따른 대나무(왕대) 추출물의 화학성분 및 생리활성" 한국식품과학회 37 (37): 542-548, 2005

4 Pryde, S. E., "The microbiology of butyrate formation in the human colon" 217 : 133-139, 2002

5 Whitman, W. B., "The methanogenic bacteria. The prokaryotes : a handbook on the biology of bacteria : ecophysiology, isolation, identification, applications. Springer-Verlag" Springer-Verlag 719-767, 1992

6 Benzie, I. F., "The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay" 239 (239): 70-76, 1966

7 Meale, S. J., "Strategies to reduce greenhouse gases from ruminant livestock" 62 (62): 199-211, 2012

8 Licitra, G., "Standardization of procedures for nitrogen fractionation of ruminant feeds" 57 : 347-358, 1996

9 SAS, "SAS/STAT Software for PC. Version 9.2" SAS Institute 2011

10 Stewart, C. S, "Rumen Microbial Metabolism and Ruminant Digestion" INRA Editions 15-26, 1991

11 Getachew, G., "Relationships between chemical composition, dry matter degradation and in vitro gas production of several ruminant feeds" 111 (111): 57-71, 2004

12 Smith, P., "Policy and technological constraints to implementation of greenhouse gas mitigation options in agriculture" 118 (118): 6-28, 2007

13 Busquet, M., "Plant extracts affect in vitro rumen microbial fermentation" 89 (89): 761-771, 2006

14 Kim, M. J., "Physiological and antibacterial activity of bamboo (Sasa coreana Nakai) leaves" 25 (25): 135-142, 1996

15 Li, X., "Phylogenetic diversity of cultivable butyrate-producing bacteria from pig gut content and feces" 94 (94): 377-381, 2016

16 AOAC, "Official Methods of Analysis of AOAC INTERNATIONAL" 2012

17 Van Soest, P. V., "Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition" 74 (74): 3583-3597, 1991

18 Goel, G., "Methane mitigation from ruminants using tannins and saponins" 44 (44): 729-739, 2012

19 Johnson, K. A., "Methane emissions from cattle" 73 (73): 2483-2492, 1995

20 Bodas, R., "Manipulation of rumen fermentation and methane production with plant secondary metabolites" 176 (176): 78-93, 2012

21 Bodas, R., "In vitro screening of the potential of numerous plant species as antimethanogenic feed additives for ruminants" 145 (145): 245-258, 2008

22 M. Joch, "In vitro Screening of Essential Oil Active Compounds for Manipulation of Rumen Fermentation and Methane Mitigation" 아세아·태평양축산학회 29 (29): 952-959, 2016

23 Bergman, E. N, "Energy contributions of volatile fatty acids from the gastrointestinal tract in various species" 70 (70): 567-590, 1990

24 Scheppach, W, "Effects of short chain fatty acids on gut morphology and function" 35 (35): S35-S38, 1994

25 Broudiscou, L., "Effects of dry plant extracts on fermentation and methanogenesis in continuous culture of rumen microbes" 87 (87): 263-277, 2000

26 Broudiscou, L., "Effects of dry plant extracts on feed degradation and the production of rumen microbial biomass in a dual outflow fermenter" 101 (101): 183-189, 2002

27 Wu, J., "Effects of copper-phosphorous salt treatments on green colour protection and fastness of ma bamboo (Dendrocalamus latiflorus)" 78 (78): 379-384, 2002

28 이신자, "Effects of Synbiotics Containing Anaerobic Microbes and Prebiotics on In vitro Fermentation Characteristics and In situ Disappearance Rate of Fermented-TMR" 아세아·태평양축산학회 24 (24): 1577-1586, 2011

29 Kato, S., "Effects of Na-butyrate supplementation in milk formula on plasma concentrations of GH and insulin, and on rumen papilla development in calves" 211 (211): 241-248, 2011

30 김언태, "Effects of Flavonoid-rich Plant Extracts on In vitro Ruminal Methanogenesis, Microbial Populations and Fermentation Characteristics" 아세아·태평양축산학회 28 (28): 530-537, 2015

31 Makkar, H. P. S, "Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds" 49 (49): 241-256, 2003

32 Hiltner, P., "Effect of soluble carbohydrates on digestion of cellulose by pure cultures of rumen bacteria" 46 (46): 642-648, 1983

33 Korea Forest Research Institute, "Distribution status of bamboo forest resources in Korea" 16-27, 2016

34 Hu, C. J., "Contents of the total flavonoids and the total phenols and antioxidant activities in the leaf from different species of Phyllostachys" 343 : 1103-1108, 2012

35 Park, Y. K., "Comparison of the flavonoid aglycone contents of Apis mellifera propolis from various regions of Brazil" 40 (40): 97-106, 1997

36 Singleton, V. L., "Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents" 16 (16): 144-158, 1965

37 Hoover, W. H, "Chemical factors involved in ruminal fiber digestion" 69 (69): 2755-2766, 1986

38 Li, X. B., "Chemical changes with maturation of the bamboo species Phyllostachys pubescens" 19 (19): 6-12, 2007

39 Flachowsky, G, "Carbon-footprints for food of animal origin, reduction potentials and research need" 39 (39): 2-14, 2011

40 Patra, R. C., "Biochemical profile of rumen liquor, blood and urine in experimental acidosis in sheep" 19 (19): 177-180, 1996

41 Re, R., "Antioxidant activity applying an improved ABTS radical cation decolorization assay" 26 (26): 1231-1237, 1999

42 Cushnie, T. T., "Antimicrobial activity of flavonoids" 26 (26): 343-356, 2005

43 McDonald, P., "Animal nutrition" Pearson education 2002

44 Theodorou, M. K., "A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds" 48 (48): 185-197, 1994