Halothane, Enflurance 및 Isoflurane 이 생쥐 2세포기 배아의 발전에 미치는 효과 = Comparative Effects of Halothane, Enflurane and Isoflurane on the Mouse Two-cell Embryo Development

다국어 초록 (Multilingual Abstract)

Background: We have previously demonstrated the isofluranc and halothane may be detrimetal to in bitro fertilization of mouse oocytes in high concentrations. The aim of this study is to compare the toxic effects of volatile anesthetics on mouse embryos using in vitro growth model of two cell mouse embryos.
Methods: mouse two-cell embryos exposed to three bolatile anesthetics, enflurane(0.5 mM; 1.5 mM), isoflurane(0.26 mM; 0.78 mM) and halothane(0.24 mM; 0.72mM). Mouse two-cell embryos unexposed to any drugs were included as controls.
Results: The percentages of two-cell mouse embryos developed over morula stages on the third day after exposure of high concentrations of isoflurane and halothane decreased significantly compared with controls. The rates of embryos arrested at 2∼8 cell stage in these groups were significantly higher than that of controls. There were no significant differences in these raters between enflurane group, isoflurane and halothane group of lower concentrations and controls. The hatching and/or hatched blastocysts development were significantly lower in isoflurane and halothane group than in controls. No significant differences in the hatching rate of blastocyst developed were observed among groups.
Conclusions: Our data show that isoflurane and halothane in high concentrations have harm effects of the in vitro growth of two cell mouse embryos. (korean J Anestbesiol 1996; 30; 245∼251)