Partitioning of Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor (hGM-CSF) from Plant Cell Suspension Culture in PEG/Sodium Phosphate Aqueous Two-phase Systems

다국어 초록 (Multilingual Abstract)

Partitioning of human granulocyte-macrophage colony stimulating factor (hGM-CSF) was achieved in the aqueous two-phase systems (ATPSs) using a crude extract of transgenic tobacco cell suspension culture. This study examined the effects of polyethylene glycol (PEG) molecular weight and concentration and the effects of sodium phosphate concentration in different PEG/sodium phosphate systems on the partition coefficient, K. The best ATPS system was 5% PEG 8,000/1.6 M sodium phosphate after 2 h of incubation at room temperature. In this system, hGM-CSF was partitioned in the PEG-rich phase with a yield of 57.99% and KhGM-CSF of 8.12. In another system, 3% PEG 10,000/1.6 M sodium phosphate, hGM-CSF was also partitioned primarily in the top phase with a yield of 45.66% and KhGM-CSF of 7.64 after 2 h of incubation at room temperature.

참고문헌 (Reference)

1 "The granulocyte-macrophage colony-stimulating factors. Science 229" 16-22, 1985

2 "The effect of osmotic pressure on production of recombinant hu-man granulocyte-macrophage colony stimulating factor in plant suspension culture." 30 : 768-773, 2002

3 "Secretion of biologically active human interleukin-2 and interleukin-4 from genetically modi-fied tobacco cells in suspension culture." 13 : 45-52, 1998

4 "Production of new/ modified proteins in transgenic plants" 10 : 163-168, 1999

5 "Pro-duction and characterization of biologically active human GM-CSF secreted by genetically modified plant cells" 19 : 131-138, 2000

6 "Physio-logical and biochemical characterization of a suspension cul-ture system for sustained exponential growth of Nicotiana syl-vestris. Physiol. Plant. 74" 1-10, 1988

7 "Physio-logical and biochemical characterization of a suspension cul-ture system for sustained exponential growth of Nicotiana syl-vestris. Physiol. Plant. 74" 1-10, 1988

8 "Phase equilib-rium and protein partitioning in aqueous mixtures of maltodextrin with polypropylene glycol." 46 : 267-274, 2001

9 "Particle fractionation in liquid two-phase systems. Biochim. Biophys. Acta. 27" 378-395, 1958

10 "Partial purification of penicillin acylase from Escherichia coli in poly(ethylene glycol)-sodium citrate aque-ous two-phase systems." B734 : 15-22, 1999

11 "Increased production of the human granulocyte-macrophage colony stimulating factor by addition of stabiliz-ing polymer in plant suspension cultures" 96 : 205-212, 2002

12 "Granulo-cyte-colony-stimulating factor (G-CSF) Role in relationships in infectious diseases." 172 : 1061-1075, 1995

13 "Foreign protein production in plant tissue cultures." 11 : 199-204, 2000

14 "Extractive cultivation of Lactococcus lactis using a poly / MgSO4?7H2O aqueous two-phase systems to produce nisin." 22 : 843-847, 2000

15 "Expression of murine GM-CSF in recombinant Aspergillus niger" 10 : 287-292, 2000

16 "Expression of human granu-locyte-macrophage colony-stimulating factor gene in insect cells by a baculovirus vector. FEBS Lett. 259" 249-253, 1990

17 "Expression and purification of native human granulocyte-macrophage colony-stimulating factor from an Escherichia coli secretion vector. DNA 6" 221-229, 1987

18 "Control of granulocytes and macrophages" 254 532-533, 1991

19 "Characterization of the mi-croheterogenetics of PIXY321 a genetically engineered granu-locyte-macrophage colony-stimulating factor/interleukin-3 fusion protein expressed in yeast." 251 : 812-820, 1998

20 "Aqueous two-phase The system of choice for extractive fermentation." 54 : 476-486, 2000

21 "A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15" 473-497, 1962

22 "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein-dye binding. Anal. Biochem. 72" 248-254, 1976