Some 20 precursor tRNAs were isolated from yeast cell, SUP 7 Mod II-3B by two-dimensional polyacrylamide gel electrophoresis. These precursors were in the 4.5 S∼5.8 S size range and contained already moidified miner bases at precursor level. Some of...
Some 20 precursor tRNAs were isolated from yeast cell, SUP 7 Mod II-3B by two-dimensional polyacrylamide gel electrophoresis. These precursors were in the 4.5 S∼5.8 S size range and contained already moidified miner bases at precursor level. Some of the precursor tRNAs were identified to have an intervening sequence in the anticodon region. The treatment of precursor tRNA with S-30 fraction and nuclei fraction gave rise to half-sized tRNA fragments and the fragments cf about 20 nucleotides long. This result suggests that tRNA processing enzyme activity which removes the intervening sequence is present in the S-30 fraction as well as in the nuclei fraction.