Establishment of Cryopreservation of Leopard Cat Semen Collected by Electro-ejaculation Method

다국어 초록 (Multilingual Abstract)

The aim of this study was to evaluate the post-thawed characteristics of leopard cat semen. In this experiment,semen was collected from two leopard cats (A and B) at wild animal center in Seoul Grand Park in Korea. After collection, the sperms were washed with D-PBS and diluted by the freezing medium (Irvine science, USA) and stored in liquid nitrogen. The post-thawed concentration was 357 × 10^6sperms/ml for A and 97 × 10^6 sperms/ml for B. The viability of post-thawed sperm from A and B individual was 24.0% and 19.0%, respectively. Pre-freezing motility of A and B individual semen was 68.54% and 56.65. Leopard cat A had more normal sperm than that of B (69.5%vs. 54.5%). Acrosome integrity analysis detected live (14.5% vs. 9.0%), damage (39.0% vs. 44.0%) and dead (46.0%vs. 47.0%) in leopard cat A and B, respectively. The present results concluded that leopard cat semen can be collected successfully by electro-ejaculation method and cryopreserved successfullyfor future use in different assisted reproductive technologies. The cryopreservation protocol needs to be modified for increasing post-thawed viability of leopard cat spermatozoa

참고문헌 (Reference)

1 하아나, "고양이의 정액 채취 방법이 동결 정액의 생존성에 미치는 영향" 한국동물번식학회 35 (35): 55-60, 2011

2 Howard JG, "Zona pellucida filtration of structurally abnormal spermatozoa and reduced fertilization in teratospermic cats" 49 : 131-139, 1993

3 Silva RC, "Testis stereology, seminiferous epithelium cycle length, and daily sperm production in the ocelot (Leopardus pardalis)" 73 : 157-167, 2010

4 Howard JG, "Teratospermic and normospermic domestic cats: Ejaculate traits, pituitary-gonadal hormones, and improvement of spermatozoal motility and morphology after swim-up processing" 11 : 204-215, 1990

5 Axner E, "Sperm morphology in the domestic cat, and its relation with fertility: A retrospective study" 42 : 282-291, 2007

6 Sontakke SD, "Semen characteristics, cryopreservation, and successful artificial insemination in the Blue rock pigeon (Columba livia)" 62 : 139-153, 2004

7 Jayaprakash D, "Semen characteristics of the captive Indian leopard, Panthera pardus" 22 : 25-33, 2001

8 공일근, "Semen characteristics of genetically identical male cats cloned via somatic cell nucleus transfer" ELSEVIER SCIENCE INC 73 (73): 638-644, 2010

9 Wildt DE, "Reproductive physiology of the clouded leopard: I. Electroejaculates contain high proportions of pleiomorphic spermatozoa throughout the year" 34 : 937-947, 1986

10 Platz CC, "Pregnancy in the domestic cat after artificial insemination with previously frozen spermatozoa" 52 : 279-282, 1978

11 Tamada T, "Molecular diversity and phylogeography of the Asian leopard cat, Felis bengalensis, inferred from mitochondrial and Y-chromosomal DNA sequences" 25 : 154-163, 2008

12 Baudi DL, "Influence of cooling rate on the ability of frozen-thawed sperm to bind to heterologous zona pellucida, as assessed by competitive in vitro binding assays in the ocelot (Leopardus pardalis) and tigrina (Leopardus tigrinus)" 69 : 204-211, 2008

13 Yin XJ, "In vitro production and initiation of pregnancies in inter-genus nuclear transfer embryos derived from leopard cat (Prionailurus bengalensis) nuclei fused with domestic cat (Felis silverstris catus) enucleated oocytes" ELSEVIER SCIENCE INC 66 : 275-282, 200607

14 Lee HS, "Enhanced histone acetylation in somatic cells induced by a histone deacetylase inhibitor improved inter-generic cloned leopard cat blastocysts" ELSEVIER SCIENCE INC 74 (74): 1439-1449, 2010

15 Howard J, "Ejaculate-hormonal traits in the leopard cat (Felis bengalensis) and sperm function as measured by in vitro penetration of zona-free hamster ova and zona-intact domestic cat oocytes" 26 : 163-174, 1990

16 Zambelli D, "Effects of ketamine or medetomidine administration on quality of electroejaculated sperm and on sperm flow in the domestic cat" 68 : 796-803, 2007

17 Carter KK, "Effects of electro-ejaculation and ketamine-HCl on serum cortisol, progesterone, and testosterone in the male cat" 5 : 431-437, 1984

18 CITES, "Convention on international trade in endangered species of wild flora and fauna, part of the endangered species act (PL 93-205, 93th Congress) and in 50 appendices"

19 Shivaji S, "Conservation of wild animals by assisted reproduction and molecular marker technology" 241 : 710-723, 2003

20 Mota PC, "Comparison between different markers for sperm quality in the cat: Diff-Quik as a simple optical technique to assess changes in the DNA of feline epididymal sperm" 65 : 1360-1375, 2006

21 Scott P, "Cats in Hafez ES, editors. Reproduction and Breeding Techniques for Laboratory Animals" Lea and Febiger 205-, 1970

22 Bader H, "Case studies in stallions with fertility problems: endocrine and spermatological aspects" 367-, 1988

23 Wildt DE, "A comparative analysis of ejaculate and hormonal characteristics of the captive male cheetah, tiger, leopard, and puma" 38 : 245-255, 1988