Metabolites of toluene, such as benzoic acid and hippuric acid were analyzed by gas chromatography. Urinary benzoic acid and hippuric acid obtained from toluene inhaled rat were extracted with ethyl acetate after acidification with hydrochloric acid.
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Metabolites of toluene, such as benzoic acid and hippuric acid were analyzed by gas chromatography. Urinary benzoic acid and hippuric acid obtained from toluene inhaled rat were extracted with ethyl acetate after acidification with hydrochloric acid.
The recovery of benzoic acid and hippuric acid in urine were over 97%, and standard calibration curves was linear in wide range with high correlation coefficients.