경남지역에서 분리한 Salmonella Enteritidis의 항생제 감수성 검사 및 random amplification polymorphic DNA (RAPD)-PCR을 이용한 유전형 분석 = Analysis of antibiotic susceptibility of Salmonella Enteritidis isolated from Gyeongnam province and the bacterial genotyping by using RAPD-PCR

다국어 초록 (Multilingual Abstract)

Salmonella Enteritidis (S. Enteritidis) are found in animals, humans, and environment. In addition, S. Enteritidis draws attention to the public health concerns due to carriage of antibiotic resistance traits. For these reasons, the prevalence and antibiotic resistance patterns of S. Enteritidis are significant issues with regard to public health. To address this issues, a total of 24 strains of S. Enteritidis from 164 samples collected from several slaughterhouses in Gyeong-Nam province in order for antibiotic resistance profiles. Subsequently, we characterized the genotyping by random amplification polymorphic DNA (RAPD)-PCR. As a result, very high level of resistance to protein synthesis inhibition antibiotics and most isolates were susceptible to others. Six random primers were used for RAPD-PCR to reveal genotypes of S. Enteritidis isolates. One of the primer, P1245, generated 147 distinct RAPD-PCR fragments ranging from 400∼3000 bp. The number of RAPD-PCR products ranged from 4 to 8 for this primer. The RAPD-PCR fragments could be placed these strains into 3 subgroups and 2 classes by UPGMA cluster analysis. Interestingly, several S. Enteritidis that isolated from different slaughterhouses showed same genotype. These results showed only limited genetic variation among the isolates, those were grouped into a few different patterns of antibiotic resistance.

참고문헌 (Reference)

1 Baumler AJ, "Tracing the origins of Salmonella outbreaks" 287 : 50-52, 2000

2 Fluit AC, "Towards more virulent and antibiotic-resistant Salmonella?" 43 : 1-11, 2005

3 Page R.D.M., "TREEVIEW: an application to display phylo-genetic trees on personal computers" 12 : 357-358, 1996

4 Popoff MY, "Supplement 2001 (no. 45) to the Kauffmann-White scheme" 154 : 173-174, 2003

5 McQuiston JR, "Sequencing and comparative analysis of flagellin genes fliC, fljB, and flpA from Salmonella" 42 : 1923-1932, 2004

6 Mikolajczyk A, "Salmonella spp. on chicken carcasses in processing plants in Polnad" 65 : 1476-1479, 2002

7 Betancor L, "Random amplified polymorphic DNA and phenotyping analysis of Salmonella enterica serovar Enteritidis isolates collected from humans and poultry in Ureguay from 1995 to 2002" 42 : 1155-1162, 2004

8 Mrema N, "Prevalence of Salmonella in raw minced meat, raw fresh sausages and raw burger patties from retail outlets in Gaborone, Botswana" 17 : 207-212, 2006

9 Bajaj BK, "Prevalence and antibiotic resistance profiles of Salmonella spp. in poultry eggs" 40 : 682-684, 2003

10 Thung TY, "Prevalence and antibiotic resistance of Salmonella enteritidis and Salmonella typhimurium in raw chicken meat at retail markets in Malaysia" 95 : 1888-1893, 2016

11 Angulo FJ, "Origins and consequences of antimicrobial-resistant nontyphoidal Salmonella: implications for the use of fluoroquinolones in food animals" 6 : 77-83, 2000

12 Garneau-Tsokikova S, "Mechanisms of resistance to aminoglycoside antibiotics: overview and perspectives" 7 (7): 11-27, 2016

13 Bajaj BK, "Incidence of Salmonella in poultry and meats and growth inhibition of Salmonella enteritidis by organic acids" 40 : 556-558, 2003

14 Carraminana JJ, "High preva- lence of multiple resistance to antibiotics in Salmonella serovars isolated from a poultry slaughterhouse in Spain" 104 : 133-139, 2004

15 Pavlicek A, "FreeTree-freeware program for construction of phylogenetic trees on the basis of distance data and bootstrap/jackknife analysis of the tree robustness. Application in the RAPD analysis of genus Frenkelia" 45 : 97-99, 1999

16 Byrd JA, "Evaluation of Salmonella serotype distributions from commercial broiler hatcheries and grower houses" 43 : 39-47, 1999

17 Rasschaert G, "Comparison of five repetitive-sequence-based PCR typing methods for molecular discrimination of Salmonella enterica isolates" 42 : 3615-3623, 2005

18 Miyamoto T, "Application of randomly amplified polymorphic DNA analysis for detection of Salmonella spp. in foods" 61 : 785-791, 1998

19 Lin AW, "Application of random amplified polymorphic DNA analysis to differentiate strains of Salmonella enteritidis" 34 (34): 870-876, 1996

20 White DG, "Antimicrobial resistance of food borne pathogen" 4 : 405-412, 2002

21 Soto SM, "Antimicrobial resistance in clinical isolates of Salmonella enterica serotype Enteritidis: relationships between mutations conferring quinolone resistance, integrons, plasmids and genetic types" 51 : 1287-1291, 2003

22 Nastasi A, "Antimicrobial resistance in Salmonella enteritidis, Southern Italy, 1990-1998" 6 (6): 401-403, 2000

23 Bauer AW, "Antibiotic susceptibility testing by a standardized single disk method" 45 (45): 493-496, 1996