AhR 발현을 통한 콤부차 분획물의 안티폴루션 효과 연구 = A Study on the Anti-Pollution Effect of Kombucha Fraction through AhR Expression

국문 초록 (Abstract)

본 연구에서는 설탕을 넣은 녹차나 홍차에 유익균을 첨가해 발효시킨 음료인 콤부차를 컬럼 크로마토그래피를 이용해 분획한 후, TLC를 통해 플라보노이드류인 퀘르세틴 글리코사이드(quercetin glycoside)의 유효성분 유무를 확인하고 그 분획물을 이용하여 공해로부터 피부를 보호 및 개선하는 안티폴루션 효과를 확인하였다. 인간 각질형성세포에 Kombucha-quercetin glycoside (K-QG)를 처리하여 세포 생존율을 확인한 결과, 100 ㎍/mL 농도까지 90% 이상의 생존율을 보였으며, 안티폴루션 효과를 보기 위해 benzo[e]pyrene과 미세먼지 자극에 의한 세포 생존율을 측정한 결과, 100 ㎍/mL에서 각각 68.79%, 50.68%의 개선율을 보였다. 또한, benzo[a]pyrene에 의해 활성화되는 aryl hydrocarbon receptor (AhR) 발현을 웨스턴 블롯을 통해 확인한 결과, 대조군에 대비하여 100 ㎍/mL에서 31.08%의 억제율을 보였다. 본 연구의 결과를 통해 K-QG는 벤조피렌과 미세먼지로부터 자극받은 피부를 보호, 개선해주며 안티폴루션 기능성 소재로써 가치가 있는 것으로 사료된다.

다국어 초록 (Multilingual Abstract)

In this study, Kombucha, a fermented drink by adding beneficial bacteria to green tea or black tea with sugar, was classified using column chromatography, and the presence or absence of quercetin glycoside, a type of flavonoids, was confirmed through TLC. In addition, the anti-pollution effect of protecting and improving the skin from pollution was confirmed using the fraction. Keratinocytes were treated with K-QG to confirm the cell viability, showing a survival rate of 90% or more up to a concentration of 100 ㎍/mL, and the cell viability by benzo[e]pyrene and fine dust stimulation to see the anti-pollution effect was measured. At 100 ㎍/mL, the improvement rates were about 68.79% and 50.68%, respectively. In addition, as a result of confirming the expression of AhR activated by benzo[a]pyrene through Western blot, it showed an inhibition rate of about 31.08% at 100 ㎍/mL compared to the control group. Through the results of this study, K-QG is thought to be valuable as an anti-pollution functional material, protecting and improving skin irritated from benzopyrene and fine dust.

참고문헌 (Reference)

1 Danielle L. St-Pierre, Maine Univ 2019

2 김재영, "콤부차의 조성과 기능성에 관한 고찰" 한국산업식품공학회 24 (24): 1-14, 2020

3 황인창, "서울시 미세먼지 관리 정책의 성과와 한계" 한국정책학회 27 (27): 27-50, 2018

4 최종규, "미세먼지의 질병에 미치는 유해성" 한국생명과학회 30 (30): 191-201, 2020

5 안은주, "미세먼지 PM10이 피부각질형성세포와 피부섬유아세포에 미치는 세포생존율 분석" 한국미용학회 25 (25): 822-826, 2019

6 C. Gotz, "Xenobiotic metabolism capacities of human skin in comparison with a 3D epidermis model and keratinocytebased cell culture as in vitro alternatives for chemical testing : Phase II enzymes" 21 (21): 364-, 2012

7 R. V. Malbasa, "TLC analysis of some phenolic compounds in Kombucha beverage" 35 : 199-, 2004

8 R. Vijayaraghavan, "Subacute(90 days)oral toxicity studies of Kombucha tea" 13 (13): 293-, 2000

9 F. Masutaka, "Role of AhR/ARNT system in skin homeostasis" 306 (306): 769-, 2014

10 C. Esser, "Functions of the aryl hydrocarbon receptor in the skin" 35 (35): 677-, 2013

11 J. Carmichael, "Evaluation of a tetrazolium-based semiautomated colorimetric assay : Assessment of chemosensitivity testing" 47 (47): 936-, 1987

12 S. C. Chu, "Effects of origins and fermentation time on the antioxidant activities of Kombucha" 98 (98): 502-, 2006

13 World Health Organization, "Air Quality Guidelines, 2nd Ed"

14 K. Higashisaka, "A sian d ust particles induce macrophage inflammatory responses via mitogen-activated protein kinase activation and reactive oxygen species production" 2014 : 2014

15 M. K. Kim, "A ir pollution and skin health : Recent studies" 20 (20): 15-, 2018