배발생 세포 관련 유전자 분석 = Cloning of Genes specifically expressed in embryogenic cells

다국어 초록 (Multilingual Abstract)

Both embryogenic(EC) and nonembryogenic calli(NEC) derived from the matured seed embryo of rice (Oryza sativa L. cv Donggin) were analyzed for differences in their gene expression. Two partial cDNA of an embryogenic cell specific gene was cloned by differential display of amplified cDNAs using a 12-mers synthetic nucleotide as a single primer. The expression of these clones, designated as REC1 and REC2, respectively, was confirmed by northern blot analysis that high levels expression only in the embryogenic cells. The 250 base of the cDNA clone REC1 have no homology with known genes. The cDNA nucleotide sequenced of the clone REC2 which is 649 base in length, was identified as a partial sequence of genes in Alcaligenes eutrophus. The partial amino acid sequence deduced from the clone REC2 showed 75 to 82 % homology with cobalt-nickel resistant proteins of Alcaligenes eutrophus.

목차 (Table of Contents)

• Ⅰ. 서론
• Ⅱ. 재료 및 방법
• Ⅲ. 결과 및 고찰
• Ⅳ. 요약
• 참고문헌