기니이픽 장관의 c-Kit 및 NK 1R 면역반응 세포구조에 대한 공초점 주사현미경적 연구 = Confocal Microscopic Observation of c-Kit- and NK1R-immunoreactive Cellular Network in Guinea Pig Intestine

다국어 초록 (Multilingual Abstract)

Background and Objectives: Immunolabelling of interstitial Cajal(IC) cells in the intestinal wall has recently been developed by using a specific marker, the anti-c-Kit antibody. Substance-P is a well-known neurotransmitter in the gastro-intestinal tract. Since the gastro-intestinal wall structures have already been well documented in the guinea pig, immunohistochemistry was done for the c-Kit-positive IC network and substance-P receptor(NK1R) in an attempt to provide a morphological basis for the mechanism regulating gastro-intestinal movement. Materials and Methods: Cryosection and whole-mount preparations of guinea pig small intestine and colon were single and double immunolabelled using the anti-c-Kit and NK1R antibodies. Immunolabelled specimens were observed under a confocal laser scanning microscope. Results : According to a three dimensional reconstruction study, it was found that (1) the c-Kit-positive celluar networks were widely distributed in the intestinal wall, (2) c-Kit-positive celluar networks encircled the ganlion, with strands in reticular configurations, and (3) the c-Kit-positive cells showed colocalization with NK1R in circular muscle(CM), not myenteric plexus(MY). Conclusion: The charateristic profiles of IC containing c-Kit-positive celluar networks and the relationship between c-Kit-positive and NK1R-positive structures provide a morphological basis upon the mechanism regulating gastro-intestinal motility.

목차 (Table of Contents)

• 서론
• 재료 및 방법
• 실험동물
• 면역조직화학염색
• (1) 냉동조직절편
• (2) Whole-mount 조직표본
• 공초점 주사현미경
• 결과
• 1. c-Kit에 대한 면역형광반응 (c-Kit-LI)
• 회장
• 결장
• 2. c-Kit와 NK1R의 이중면역형광염색반응 (c-Kit-LI, NK1R-LI)
• 회장
• 결장
• 동일세포내 c-Kit-LI와 NK1R-LI 의 공존(colocalization)
• 고찰
• 결론