식물생장억제제의 분리와 동정을 위하여 500여 종의 토양미생물로부터 상추 유묘에 대하여 강한 저해활성을 나타내는 미생물 균주 GA75-1을 선발하였다. 이 균주는 16S rRNA의 핵산염기서열 분...
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https://www.riss.kr/link?id=A76508011
2009
English
525
KCI등재,SCIE,SCOPUS
학술저널
319-324(6쪽)
0
0
상세조회0
다운로드국문 초록 (Abstract)
식물생장억제제의 분리와 동정을 위하여 500여 종의 토양미생물로부터 상추 유묘에 대하여 강한 저해활성을 나타내는 미생물 균주 GA75-1을 선발하였다. 이 균주는 16S rRNA의 핵산염기서열 분...
식물생장억제제의 분리와 동정을 위하여 500여 종의 토양미생물로부터 상추 유묘에 대하여 강한 저해활성을 나타내는 미생물 균주 GA75-1을 선발하였다. 이 균주는 16S rRNA의 핵산염기서열 분석결과, Curvibacter sp.로 동정되었다. 이 균주의 배양 여액에 대하여 dichloromethane, ethyl acetate 및 butanol을 이용하여 순차적으로 분배한 다음, 강한 활성을 나타내는 butanol 가용성분획에 대하여 활성 지향형 분리를 통하여 화합물 1과 2의 두 가지 화합물을 분리하였다. 1과 2는 상추유묘의 뿌리 생장을 50㎎ L⁻¹에서 각각 56.8과 54.1% 저해하였으며, 줄기생장을 22.5 및 47.3% 저해하였다. 1과 2는 ¹H-NMR과 EI-MS 분석을 통하여 각각 uracil과 4-methoxybenzoic acid로 동정되었다. 1과 2는 50% 뿌리성장 저해농도 (IC₅₀) 값이 48.4와 49.1㎎ L⁻¹이었으며, 줄기에 대하여는 IC₅₀값이 각각 > 100.0와 51.3㎎ L⁻¹이었다. Uracil 유도체는 매우 잘 알려진 제초제 군에 속하나 uracil 자체에 대한 제초활성은 본 보고가 처음이며, 4-methoxybenzoic acid가 상추유묘에 대한 저해제로서 Curvibacter sp.에서 분리 보고된 것도 이번이 처음이다. 상기 화합물을 함유하는 Curvibacter sp. GA75-1은 식물의 도복 방지제, 또는 생물농약으로 이용될 수 있을 것으로 기대된다.
다국어 초록 (Multilingual Abstract)
The microbial strain GA75-1, which exhibited growth inhibitory activity on lettuce seedlings, was selected out of more than five hundred bacterial colonies by pot experiment for isolation and identification of plant growth retardants. The strain was i...
The microbial strain GA75-1, which exhibited growth inhibitory activity on lettuce seedlings, was selected out of more than five hundred bacterial colonies by pot experiment for isolation and identification of plant growth retardants. The strain was identified as Curvibacter sp. by the nucleotide sequence of the 16S rRNA gene. The culture broth was consecutively partitioned with dichloromethane, ethyl acetate, and butanol. The bioassay-guided fractionation of butanol extract led to the isolation of compound 1 and 2 which inhibited 56.8 and 54.1% of lettuce root elongation and 22.5 and 47.3% of shoot growth at 50 ㎎ L⁻¹, respectively. From ¹H NMR and EI-MS spectra, 1 and 2 were identified as uracil and 4-methoxybenzoic acid, respectively. The concentrations of uracil and 4-methoxybenzoic acid required for 50% inhibition of root were 48.4 and 49.1 ㎎ L⁻¹ and of shoot were > 100.0 and 51.3 ㎎ L⁻¹, respectively. Uracil derivatives are one of very well-known herbicide groups; however, this is the first report on the herbicidal effect of uracil itself on lettuce. In addition, 4-methoxybenzoic acid is firstly isolated from the genus C. lanceolatus as an inhibitor of lettuce seedling growth. C. lanceolatus containing the above compounds is expected to be useful as the lodging-preventer and/or biotic herbicide.
목차 (Table of Contents)
참고문헌 (Reference)
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1 최기영, "식물 공장에서 반결구 상추와 잎상추의 잎끝마름 발생에 미치는 온도의 영향" 한국원예학회 44 (44): 805-808, 2003
2 황현정, "상추 종자의 발아에 있어 광질 및 생장조절물질의 영향" 한국생물환경조절학회 17 (17): 51-59, 2008
3 Sherman, H, "Toxicity studies with 5-bromo-3-sec-butyl-6-methyluracil" 34 : 189-196, 1975
4 Kanbe, K., "Thienodolin, a new plant growth-regulating substance produced by a Streptomyces strain II. Structure of thienodolin" 57 : 636-637, 1993
5 Leifson, E, "The Bacterial Flora of Distilled and Stored Water. III. new species of the genera Corynebacterium, Flavobacterium, Spirillum and Pseudomonas" 12 : 161-170, 1962
6 Pouchert, C. J, "The Aldrich Library of 13C and 1H FT NMR Spectra Ed. I. vol 3" Aldrich Chemical Co. Inc. 365-, 1993
7 Fan, X.-Q., "Synthesis of 5-bromo-3-sec-butyl-6-methyluracil (bromacil)" 15 : 11-16, 2007
8 Hwang,H.-J, "Seed germination of lettuce cultivars affected by dry heat treatment" 25 (25): 2007
9 Zhang, Q., "Research progress on herbicidally active uracil derivatives" 45 : 73-77, 2006
10 Cox,R.H, "Proton magnetic resonance spectra of monosubstituted benzoic acids" 25 : 1189-1194, 1969
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21 Hoang, L., "Growth inhibitors of lettuce seedlings from Bacillus cereus EJ-121" 47 : 149-154, 2005
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Washing Efficiency of Acidic Electrolyzed Water on Microbial Reduction and Quality of ‘Fuji’ Apples
Removal of Indoor Tobacco Smoke under Light and Dark Conditions as Affected by Foliage Plants
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2023 | 평가예정 | 해외DB학술지평가 신청대상 (해외등재 학술지 평가) | |
2020-01-01 | 평가 | 등재학술지 유지 (해외등재 학술지 평가) | |
2017-02-08 | 학술지명변경 | 외국어명 : Korean Journal of Horticultural Science & Technology -> Horticultural Science & Technology | |
2011-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2009-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2007-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2004-01-01 | 평가 | 등재후보학술지 선정 (신규평가) | |
2003-01-01 | 평가 | 등재후보 1차 PASS (등재후보1차) | |
2002-07-01 | 평가 | 등재후보학술지 선정 (신규평가) |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0.92 | 0.74 | 0.83 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0.77 | 0.73 | 1.115 | 0.19 |