Evidence for Genetic Similarity of Vegetative Compatibility Groupings in Sclerotinia homoeocarpa

다국어 초록 (Multilingual Abstract)

Vegetative compatibility groups (VCGs) are determinedfor many fungi to test for the ability of fungalisolates to undergo heterokaryon formation. In severalfungal plant pathogens, isolates belonging to a VCGhave been shown to share significantly higher geneticsimilarity than those of different VCGs. In this studywe sought to examine the relationship between VCGand genetic similarity of an important cool season turfgrasspathogen, Sclerotinia homoeocarpa. Twenty-twoS. homoeocarpa isolates from the Midwest and EasternUS, which were previously characterized in severalstudies, were all evaluated for VCG using an improvednit mutant assay. These isolates were also genotypedusing 19 microsatellites developed from partial genomesequence of S. homoeocarpa. Additionally, partial sequencesof mitochondrial genes cytochrome oxidase IIand mitochondrial small subunit (mtSSU) rRNA, andthe atp6-rns intergenic spacer, were generated for isolatesfrom each nit mutant VCG to determine if mitochondrialhaplotypes differed among VCGs. Of the 22isolates screened, 15 were amenable to the nit mutantVCG assay and were grouped into six VCGs. The 19microsatellites gave 57 alleles for this set. Unweightedpair group methods with arithmetic mean (UPGMA)tree of binary microsatellite data were used to producea dendrogram of the isolate genotypes based on microsatellitealleles, which showed high genetic similarityof nit mutant VCGs. Analysis of molecular variance of microsatellite data demonstrates that the currentnit mutant VCGs explain the microsatellite genotypicvariation among isolates better than the previous nitmutant VCGs or the conventionally determined VCGs.
Mitochondrial sequences were identical among all isolates,suggesting that this marker type may not be informativefor US populations of S. homoeocarpa.

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