內耳組織의 Na^+, K^+ ATPase 分布와 알부민輸送에 關한 超微形態學的 硏究 = An Ultrastructural Study on the Distribution of Na^+, K^+ -ATPase and Transcytosis of Albumin in the Cochlea

다국어 초록 (Multilingual Abstract)

This study was carried out to investigate distribution of Na^+, K^+ -ATPase in the cochlear tissues by means of electron microscopic immunocytochemistry and transcytosis of albumin in the cochlear tissues. Ten cochleas obtained from guinea pigs were stained by avidin-biotin-peroxidase with anti-human Na^+, K^+ -ATPase rabbit IgG as primary antibody. Three guinea pigs were perfused with colloidal gold binding bovine serum albumin. Four were injected into the endolymphatic and perilymphatic spaces via oval and round windows. The cochlear tissues were observed with electron microscope. The results obtained were were as follows.
Na^+, K^+ -ATPase was predominently distributed in basolateral infoldings and mitochondrial membranes and cristae, and moderately in the cytoplasmicn membrane and interdental cells.
Albumin was distributed on the endothelial cells of the strial capillaries and surrounding interstitium. There were no albumins in marginal cells, intermediate cells, basal cells, Reissner' s membrane, and cells of the organ of Corti.
According to the above results. it is suspected that transport of Na^+ and K^+ is performed in basolateral infoldings of marginal cells by active transport mechanism, and that immuncoytochemical method is more excellent to demonstrate Na^+, K^+ -ATPase than that of enzyme histochemistry.
Transcytosis of albumin into the endolymphatics may be restricted by stria vascularis, Reissner' s membrane, and the cells of the organ of Corti.