약용식물 추출물의 Helicobacter pylori에 대한 항균 활성과 항산화 활성에 미치는 효과 = The Effect of Medicinal Herb Extract on Antimicrobial Activity against Helicobacter pylori and Antioxidant Activity

다국어 초록 (Multilingual Abstract)

This study was designed to investigate the effect of medicinal herb extract on the antioxidant and antimicrobial activities against Helicobacter pylori, which is known as a ulcerogenic pathogen. The concentration of total phenolic compound of Scutellaria baicalensis(13.14%) was highest among water extracts and that of Ulmus parvifolia(15.12%) was highest among the ethanol extracts. The antioxidant activity of the water extract of Scutellaria baicalensis and of the ethanol extract of Ulmus parvifolia were 91.00% and 65.03%, respectively, in DPPH assay. The antioxidant activity of the water extract of Scutellaria baicalensis and of the ethanol extract of Ulmus parvifolia were 32.90% and 27.70%, respectively, in SOD assay. The antioxidant activity of the water extract of Ulmus parvifolia and of the ethanol extract of Glycyrrhiza uralensis Fischer was 2.15 and 2.17, respectively, in TBARS assay. In disc method, Scutellaria baicalensis showed the highest anti-microbial activity against H. pylori, followed by Glycyrrhiza uralensis Fischer among the water extracts and Glycyrrhiza uralensis Fischer showed the highest anti-microbial activity followed by Radix puerariae among ethanol extract.

참고문헌 (Reference)

1 김은영, "항산화활성을 나타내는 약용식물 소재 탐색" 한국식품과학회 36 (36): 333-338, 2004

2 박영숙, "한약재 추출물의 폴리페놀 화합물과 항산화 활성" 동아시아식생활학회 12 (12): 23-31, 2002

3 박창호, "한약재 추출물에 의한 Helicobacter pylori의생장 및 Urease 활성 억제" 한국생물공학회 19 (19): 187-191, 2004

4 임정대, "한국산 약용식물로부터의 SOD 활성 및 Phenolic Compounds 함량 비교" 한국약용작물학회 12 (12): 191-202, 2004

5 손종연, "석류씨 추출물의 항산화 및 항균활성" 한국식품조리과학회 21 (21): 171-179, 2005

6 최수임, "생약재 추출물의 hyaluronidase 저해 및 라디칼 소거 활성 검색" 한국생물공학회 18 (18): 282-288, 2003

7 Cho BI, "Toxicity of oxygen free radical and clinical use of scavengers." 3 : 115-130, 1993

8 Kuhnau J, "The flavonoids ; a class of semi essential food components ; their role in human nutrition." 24 : 117-120, 1976

9 Lee JJ, "The antimicrobial activity of medicinal plants extracts against Helicobacter pylori." 31 : 764-770, 1999

10 Rhee KH, "Prevalence of Helicobacter pylori infection in Korean." 25 : 475-490, 1990

11 Huang MT, "Phenolic compounds in food. In phenolic compounds in food and their effects on health Ⅱ." Maple Press, New York. 2-7, 1992

12 Azuma K, "Phenolic antioxidants from the leaves of Corchorus olitorius L" 47 : 3963-3966, 1999

13 Gavidson PH, "Methods for testing the efficacy of food antimicrobials." 43 : 148-152, 1989

14 Marklund S, "Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase." 47 : 469-474, 1974

15 Baik SC, "Increased oxidative DNA damage in Helicobacter pylori-infected human gastric mucosa." 56 : 1279-1282, 1996

16 Tabak M, "In vitro inhibition of Helicobacter pylori by extracts of thyme." 80 : 667-672, 1996

17 Hentschel E, "Effect of ranitidine and amoxicillin plus metronidazole on the eradication of Helicobacter pylori and the recurrence of duodenal ulcer." 328 : 308-312, 1993

18 Kim JD, "Development of antioxidant tea for anti-aging." 14 : 401-409, 1999

19 Yee YK, "Chinese tea consumption and lower risk of Helicobacter infection." 552-555, 2002

20 Rauws EA, "Campylobacter pyloridis-associated chronic active antral gastritis: a prospective study of its prevalence and the effect of antibacterial and antiuclera treatment." 9 : 33-40, 1988

21 Higasi GS, "Appraisement of antioxidative activity from vegetable." 57 : 56-64, 2004

22 Cha BC, "Antioxidative and antimicrobial effects of nut species." 29 : 28-32, 1998

23 Cha JY, "Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata." 28 : 1310-1315, 1999

24 Blois MS, "Antioxidant determination by the use of a stable free radical." 26 : 1199-1200, 1958

25 Ham SS, "Antimutagenic effects of juices from edible Korean wild herbs" 26 : 155-161, 1997

26 Kubo JJ, "Anti-Helicobacter pylori agents from the Cashew Apple." 47 : 533-537, 1999

27 Korean Central Center Registry, "Annual report of the Korean Central Center Registry." 2003

28 Cover TL, "A bacterial cause of gastritis, peptic ulcer disease and gastric cancer." 61 : 21-26, 1995

29 Rhee KH, "A Prospective syudy on the Campylobacter pylori isolated from patients of gastroduodenal inflammatory condition." 23 : 9-16, 1988