자기공명 분석법을 이용한 백서 피판의 대사에 대한 연구 = METABOLIC STUDIES SKIN FLAPS OF RATS USING NMR SPECTROSCOPY

다국어 초록 (Multilingual Abstract)

Innumerable techniques have been used to measure, index, or predict the viability of skin flaps after elevation. However, these are largely indirect and don't accurately reflect the events that occur at kthe cellular level. A method was sought that gives direct, reproducible, and accurate data about physiological and biochemical changes that occur during flap elevation.
Because of its ability to monitor changes in the levels of high energy phosphorus metabolites(ATP, or adenosine triphosphate, PC or phosphocreatine, Pi or inorganic phosphate), 31-P magnetic resonance spectroscopy (MRS) holds promise of providing direct assessment of the metabolic status and biochemical changes that occur during skin flap elevation.
MRS monitoring was performed on three portions of raised random pattern skin flaps of 5 rats. The spectrometer used for 31-P nuclear magnetic resonance analysis was a Bruker Biospec(4.7 Tesla) system, and a modified surface coil constructed of two layers of copper coil was used.
The results have validated the ability of 31-P MRS to :
1. define the regional flxes in the levels of ATP, PCr, and Pi in skin flaps.
2. demonstrate that, of these, PCr levels represent the most sensitive indicator of flap well being.
3. measure intracellular pH through the chemical shift of the Pi resonance.
4. establish predictive correlations between profile of normal, compromised, and failing skin flaps and have thus prepared the way for noninvasive in vivo topical magnetic resonance investigations of flaps.