By noncompetitive ELISA using anti-AFP MAbs, it was confirmed that screening of serum AFP level is essential in diagnosis of the hepatocellular carcinoma. By indirect immunofluorescence technique, anti AFP MAbs were found to react with the surface of ...
By noncompetitive ELISA using anti-AFP MAbs, it was confirmed that screening of serum AFP level is essential in diagnosis of the hepatocellular carcinoma. By indirect immunofluorescence technique, anti AFP MAbs were found to react with the surface of hepatoma cells (70%) and MCF-7 breastocarcinoma cells (45%), but not with ME-180 cervix carcinoma cells. The purified anti-AFP MAbs were coupled with RTA to form immunotoxin, whose effectiveness in in vitro killing of target cells was evaluated by cytotoxicity assay. The results showed that this immunotoxin was specifically cytotoxic to hepatocellular carcinoma cells.