足三里 杜？藥鍼이 Collagen-induced arthritis에 미치는 영향 = A Study on the Effect of Herbal-acupuncture with Eucomiae Cortex Solution at Joksamni(ST36) on Collagen-induced Arthritis

다국어 초록 (Multilingual Abstract)

Objectives : The purpose of this study is to observe the effects of Eucomiae Cortex herbal-acupuncture solution(EC-HAS) at Joksamni(ST36) on arthritis of mice induced by Collagen Ⅱ.
Methods : The author performed several experimental items. First, it is the cell survival rate of mice lung fibroblasts. Second, it is the incidence rate of arthritis and arthritis index of CIA. Third, it is the levels of IL-6, TNF-α, IFN-γ, IL-1β, IgG, IgM and anti-collagen Ⅱ in serum and the level of IFN-γ, IFN-γ/IL-4 ratio in CIA mouse spleen cell culture. Fourth, it is histological analysis of the mice joint. Fifth, it is expression ratio of CD3e+ to CD19+ cell, CD4+ to CD8+ cell, CD69+/CD3e+ cells, CD11a+/CD19+ cells, CD11b+/Gr-1+ cells and CD4+/CD25+ cells.
Results & Conclusion : 1. In the EC-HA, the incidence of arthritis and arthritis index were significantly decreased.
2. In EC-HA, the levels of IL-6, IFN-γ, TNF-α, IL-1β, IgG, IgM and anti-collagen Ⅱ in serum of CIA mice and the level of IFN-γ, IL-4, IFN-γ/IL-4 ratio in CIA mouse spleen cell culture were significantly decreased.
3. In the histological study, the cartilage destruction and synovial cell proliferation were decreased in the EC-HA, and the collagen fiber expressions in the EC-HA were similar with that of the Normal group.
4. In the EC-HA, the expression ratio of CD3e+ to CD19+ cell and CD4+ to CD8+ cell were similarly maintained as Normal group in lymph nodes, and CD69+/CD3e+ cells and CD11a+/CD19+ cells were decreased in lymph nodes, and CD11b+/Gr-1+ cells and CD4+/CD25 +cells were decreased in synovium.
These results suggest that EC-HA at ST36 has an effect to control synovial cell proliferation and cartilage destruction in rheumatoid arthritis, and to be put to practical use in the future rheumatoid arthritis clinic.

참고문헌 (Reference)

1 해리슨 번역 편찬위원회, "해리슨 내과학" 도서출판정담 1179-1185, 1997

2 김전, "통증의 병태생리" 20 (20): 1-7, 2002

3 이준호, "택란약침이 collagen으로 유발시킨 관절염에 미치는 영향" 17 (17): 287-318, 2000

4 정태호, "최신면역학 강의" 경북대학교출판부 193-235, 1996

5 성은미, "적작약약침이 관절염에 미치는 실험적 연구" 17 (17): 221-50, 2000

6 이규범, "임상병리 핸드북" 고문사 233-5, 1995

7 대한약침학회, "약침제제와 임상응용2" 대한약침학회 1-5, 1997

8 대한약침학회, "약침요법 시술지침서" 대한약침학회 13-19, 1999

9 오찬호 역, "신면역학입문" 지구문화사 63-, 1997

10 강재성, "세포분자면역학 5판" 범문사 243 -53, 2004

11 이연경, "목방기약침이 관절염에 미치는 영향" 18 (18): 125-42, 2001

12 김세윤, "류마티스 진료" 군자출판사 219-, 2001

13 한창환, "류마토이드 관절염 활액막에서의 p53종양 억제 유전자 변이" 36 (36): 9-15, 2001

14 金起瑩, "類風濕性關節炎의 病因病機 및 治法에 대한 文獻的 考察" 8 (8): 223-, 1998

15 吉利和 編著, "內科診斷學" 제일의학사 176 : 668-9, 1994

16 李文鎬外, "內科學 下" 學林社 1693-1702, 1994

17 서범석, "免疫과 관련된 穴 考察" 2 (2): 133-61, 1994

18 강소신의학원편, "中藥大辭典" 정담출판사 1450-5, 1998

19 Pelman H, "Rheumatoid arthritis synovial macrophages express the Fas associated death domain like interleukin 1beta converting enzyme inhibitory protein and are refractory to Fas mediate apoptosis" 44 (44): 21-30, 2001

20 Wang H, "Regulation of autoreactive anti IgG B cells in normal and autoimmune mice" 19 (19): 259-270, 1999

21 Juarranz Y, "Protective effect of vasoactive intestinal peptide on bone destruction in the collagen induced arthritis model of rheumatoid arthritis" 7 (7): 1034-45, 2005

22 Bauer K,, "Perforin deficiency attenuates collagen induced arthritis" 7 (7): 877-884, 2005

23 Bruce albert, "Molecular biology of the cell 3rd edition" Garland Publishing 1195-7, 1994

24 Thirumalai Ramalingam, "Kinetics of Cellular Responses to Intraperironeal Brugia pahangi Infecrions in Normal and Immunodeficient Mice" 4361-7, 2003

25 Pongchairerk U, "Focal adhesion kinase and Src phosphorylations in HGF induced proliferation and invasion of human cholangiocarcinoma cell line HuCCA1" 11 (11): 5845-1 52, 2005

26 Katherine L,, "Cytokine level in synovial fluid and serum of patient with Juvennile Rheumatoid Arthritis" 21 : 2359-63, 1994

27 Robert R. Rich, "Clinical immunology" Mosby 4-, 2001