복분자와 홍삼 발효 추출물의 복합투여가 고지방 고콜레스테롤 식이를 섭취한 흰쥐의 지질대사 및 비만에 미치는 영향

국문 초록 (Abstract)

이 연구는 고지방 고콜레스테롤 식이를 12주 동안 랫트에게 공급하여 복분자와 홍삼, 복분자와 홍삼 발효 추출물의 지질대사 및 비만 개선효과를 조사하였다. 정상식이, 고지방 고콜레스테롤식이, 양성대조군, 유산균발효군, 복분자와 홍삼, 복분자와 홍삼발효 추출물 투여군의 체중증가, 음수, 식이 섭취량은 그룹간의 유의적인 차이를 보이지 않았다. 하지만 혈중 HDL-콜레스테롤은 고지방 고콜레스테롤 식이와 비교 했을 때 복분자와 홍삼 발효추출물에서 유의적인 증가를 보였으며, LDL-콜레스테롤, 중성지방은 유의적인 감소율을 보였다. 또한, 간 조직에서 복분자와 홍삼 발효추출물 처치에 의해 HMG-CoA reductase, LDL receptor 및 SREBP-2 mRNA의 발현 증가와 지방생성 억제를 확인하였다. 그리고 복분자와 홍삼 발효 추출물 투여군은 비만인자인 혈중 leptin과 FAS의 농도를 유의적으로 감소시켰을 뿐만 아니라 대변에서 체내 콜레스테롤 배출을 증가시켰다. 이러한 결과로 미루어보아 복분자와 홍삼 발효 추출물은 혈중 지질 대사 및 비만의 예방에 기여할 것으로 판단된다.

다국어 초록 (Multilingual Abstract)

This study investigated the effects of fermented black raspberry (BR) and red ginseng (RG) extract co-treatment on lipid metabolism and obesity in rats fed with a high fat/high cholesterol diet (HFHCD) for 12 weeks. Compared to the corresponding values in rats fed with a HFHCD, total cholesterol and low-density lipoprotein (LDL)-cholesterol and triglyceride levels decreased whereas high-density lipoprotein (HDL)-cholesterol levels increased in rats treated with fermented BR and RG extracts. These extracts significantly increased the expression of HMG-CoA reductase, LDL receptor, and sterol regulatory-element-binding protein-2 (SREBP-2) mRNA, but decreased the mRNA expression of SREBP-1. Additionally the serum levels of leptin and fatty acid synthase were decreased. Moreover, supplementation with fermented BR and RG effectively increased fecal cholesterol excretion. These results suggest that fermented BR and RG extracts might be effective at preventing hypercholesterolemia and obesity.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• References

참고문헌 (Reference)

1 남기열, "홍삼과 백삼의 비교 고찰" 고려인삼학회 29 (29): 1-18, 2005

2 한영오, "혈중산소포화도/맥파 신호 측정 및 무선 전송을 위한 모듈 개발" 한국전자통신학회 6 (6): 981-986, 2011

3 Sung-Jin Park, "초임계 추출 계피오일의 3T3-L1 지방전구세포의 분화 전사인자 억제에 의한 지방대사 조절" 한국생명과학회 24 (24): 510-517, 2013

4 박종혁, "전통발효식품에서 분리한 유산균의 항균 활성 및 발효유 스타터 개발" 한국식품저장유통학회 20 (20): 712-719, 2013

5 강복희, "유산균을 이용한 발효인삼의 ginsenoside 유도체 및 품질특성" 한국식품저장유통학회 20 (20): 573-582, 2013

6 이수정, "복분자 미숙과와 홍삼 추출물의 콜레스테롤 개선 효과" 한국식품과학회 45 (45): 628-635, 2013

7 최혜란, "복분자 미숙과 물추출물의 콜레스테롤 개선 효과" 한국식품영양과학회 42 (42): 1899-1907, 2013

8 김미정, "백년초 및 녹차 가루 첨가 연근 부각의 지질저하 기능성" 한국식품영양과학회 43 (43): 333-340, 2014

9 손효현, "고지방 식이를 섭취한 흰쥐에 있어서 Xylooligo당이 간의 HMG-CoA Reductase활성 및 간조직의 형태학적 변화에 미치는 영향" 한국영양학회 35 (35): 1015-1022, 2002

10 Halaas JL, "Weightreducing effects of the plasma protein encoded by the obese gene" 269 : 543-546, 1995

11 Park CK, "Trends and prospect of ginseng products in market health functional food" 40 : 30-45, 2007

12 Rossouw JE, "The effect of skim milk, yoghurt, and full cream milk on human serum lipids" 34 : 351-356, 1981

13 Goldin BR, "Survival of Lactobacillus species (strain GG) in human gastrointestinal tract" 37 : 121-128, 1992

14 Yoon SH, "Study on the preventive effect of ginsenosides against hypercholesterolemia and its mechanism" 17 : 1-12, 1993

15 Nylén ES, "Statins modulate the mortality risk associated with obesity and cardiorespiratory fitness in diabetics" 98 : 3394-3401, 2013

16 Wang SY, "Scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydroxyl radicals, and singlet oxygen" 48 : 5677-5684, 2000

17 Shimano H, "SREBPs: Physiology and pathophysiology of the SREBP family" 276 : 616-621, 2009

18 Pal S, "Red wine polyphenolics increase LDL receptor expression and activity and suppress the secretion of ApoB100 from human HepG2 cells" 133 : 700-706, 2003

19 Bae EA, "Protective effect of fermented red ginseng on transient focal ischemic rats" 27 : 1136-1140, 2004

20 Mahley RW, "Plasma lipoproteins: Apoprotein structure and function" 25 : 1277-1294, 1984

21 Agheli N, "Plasma lipids and fatty acid synthase activity are regulated by short-chain fructo-oligosaccharides in sucrose-fed insulin-resistant rats" 128 : 1283-1288, 1998

22 유기석, "Phylogenic Relationships of Rubus Species Revealed by Randomly Amplified Polymorphic DNA Markers" 한국작물학회 11 (11): 39-44, 2008

23 Behme MT, "Leptin: Product of the obese gene" 31 : 138-141, 1996

24 Lee SM, "JNK-mediated SREBP-2 processing by genistein up-regulates LDLR expression in HepG2 Cells" 4 : 308-314, 2014

25 Rao DR, "Influence of milk and themophilus milk on plasma cholesterol levels and hepatic cholesterogenesis in rats" 46 : 1339-1341, 1981

26 Perdigon G, "Immune system stimulation by probiotics" 78 : 1597-1606, 1995

27 Tanida M, "High-fat diet-induced obesity is attenuated by probiotic strain Lactobacillus paracasei ST11 (NCC2461) in rats" 2 : 159-169, 2008

28 Jeon BS, "Fermented mushroom milk-supplemented dietary fibre prevents the onset of obesity and hypertriglyceridaemia in Otsuka Long-Evans Tokushima fatty rats" 7 : 709-715, 2005

29 Pelleymounter MA, "Effects of the obese gene product on body weight regulation in ob/ob mice" 269 : 540-543, 1995

30 Kang BH, "Effect of saponin fraction of Panax ginseng C. A. Meyer on blood serum lipoprotein distribution of cholesterol fed rabbits" 10 : 114-121, 1986

31 Rim JCK, "Effect of high fat and high carbohydrate diet on serum leptin and lipids concentration in rat" 34 : 123-131, 2001

32 Vahouny GV, "Dietary fiber and lymphatic absorption of cholesterol in the rat" 31 : S208-A212, 1978

33 Salminen S, "Demonstration of safety of probiotics- a review" 44 : 93-106, 1998

34 Murase T, "Coffee polyphenols suppress diet-induced body fat accumulation by downregulating SREBP-1c and related molecules in C57BL/6J mice" 300 : E122-E133, 2011

35 Neuschwander-Tetri BA, "Clinical, laboratory and histological associations in adults with nonalcoholic fatty liver disease" 52 : 913-924, 2010

36 Chen T, "Chemopreventive properties of black raspberries in N-nitrosomethylbenzylamine- induced rat esophageal tumorigenesis: Down-regulation of cyclooxygenase-2, inducible nitric oxide synthase, and c-jun" 66 : 2853-2859, 2006

37 Ju HK, "Characterization of increased phenolic compounds from fermented Bokbunja (Rubus coreanus Miq.) and related antioxidant activity" 49 : 820-827, 2009

38 Rodríguez-Cantú LN, "Broccoli (Brassica oleracea var. italica) sprouts and extracts rich in glucosinolates and isothiocyanates affect cholesterol metabolism and genes involved in lipid homeostasis in hamsters" 59 : 1095-1103, 2011

39 Seeram NP, "Blackberry, black raspberry, blueberry, cranberry, red raspberry and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro" 9339 54 : -9329, 2006

40 Joo CN, "Biochemical study of some pharmacological effects of Panax ginseng C. A. Meyer" 13 : 63-80, 1980

41 TRINH, HIEN-TRUNG, "Bifidus Fermentation Increases Hypolipidemic and Hypoglycemic Effectsof Red Ginseng" 한국미생물·생명공학회 17 (17): 1127-1133, 2007

42 Sung, H, "Beneficial effects of a combination of Korean red ginseng and highly active antiretroviral therapy in human immunodeficiency virus type 1-infected patients" 16 : 1127-1131, 2009

43 Gilliland SE, "Assimilation of cholesterol by Lactobacillus acidophilus" 49 : 377-381, 1985

44 Yang HM, "Antiinflammatory activities of Rubus coreanus depend on the degree of fruit ripening" 22 : 102-107, 2008

45 Danielson AD, "Anticholesteremic property of Lactobacillus acidophilus yogurt fed to mature boars" 67 : 966-974, 1989

46 Miyoshi M, "Anti-obesity effect of Lactobacillus gasseri SBT2055 accompanied by inhibition of pro-inflammatory gene expression in the visceral adipose tissue in diet-induced obese mice" 53 : 599-606, 2014

47 Hai-Dan Yuan, "Anti-diabetic Effect and Mechanism of Korean Red Ginseng in C57BL/KsJ db/db Mice" 고려인삼학회 32 (32): 187-193, 2008

48 Després JP, "Abdominal obesity and metabolic syndrome" 444 : 881-887, 2006

49 남상명, "40세 이상 성인에서 비만지표가 혈중지질수준과 고지혈증 발생에 미치는 영향" 대한비만학회 17 (17): 20-28, 2008