Biochemical Characterization of Collagen from the Starfish Asterias amurensis

다국어 초록 (Multilingual Abstract)

The industrial use of bovine and porcine collagen has been expanded to include foods, cosmetics, and biomaterials. However, due to outbreaks of bovine spongiform encephalopathy and foot and mouth disease, new, alternative, safe sources of collagen are being sought. In this study, pepsinsolubilized collagen (PSC) was isolated from the tissue of a starfish (Asterias amurensis) and shown to consist of (α1)2α2 heterotrimer, which is similar to calf skin type I collagen. However, the PSC was denaturated at 24.7oC, which is about 12oC lower than mammalian collagen. Immunoblotting
assay using polyclonal anti-type I collagen antibody revealed that the starfish collagen contained similar affinity motifs. In XTT assay, PSC suspension had cell growth activity and no showed no cytotoxicity.

다국어 초록 (Multilingual Abstract)

The industrial use of bovine and porcine collagen has been expanded to include foods, cosmetics, and biomaterials. However, due to outbreaks of bovine spongiform encephalopathy and foot and mouth disease, new, alternative, safe sources of collagen are being sought. In this study, pepsinsolubilized collagen (PSC) was isolated from the tissue of a starfish (Asterias amurensis) and shown to consist of (α1)2α2 heterotrimer, which is similar to calf skin type I collagen. However, the PSC was denaturated at 24.7oC, which is about 12oC lower than mammalian collagen. Immunoblotting
assay using polyclonal anti-type I collagen antibody revealed that the starfish collagen contained similar affinity motifs. In XTT assay, PSC suspension had cell growth activity and no showed no cytotoxicity.

참고문헌 (Reference)

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