키토산 기반 삼블록 공중합체를 이용한 온도감응성 하이드로젤 제조 및 특성 분석 = Preparation and Characterization of Thermal-responsive Hydrogel UsingTriblock Copolymer Based on Chitosan

다국어 초록 (Multilingual Abstract)

This study synthesized chitosan-b-F127-b-chitosan triblock copolymer (CFC) with pluronic F127 and chitosan to increase sol-gel transition temperature. CFC triblock copolymer was synthesized by chain polymerization between F127 macromer and radical species obtained from β-1,4 glycoside bond degradation of chitosan. These physicochemical properties were analyzed by Fourier transform infrared spectroscopy (FTIR) and proton nuclear magnetic resonance (1H-NMR), which showed that CFC was successfully synthesized. The vial invert method and rheometer confirmed the sol-gel transition behavior of CFC according to temperature. As a result of observing the morphological surface of the CFC hydrogel using a field emission scanning electron microscope (FE-SEM), it was confirmed that the pore size decreased as the concentration increased. In addition, as a result of live/dead assay using human gastric adenocarcinoma (AGS) cells, it was confirmed that cells survive in CFC hydrogel, proving its potential as a cell scaffold. These results can be applied to diverse bio fields because the CFC hydrogel prepared in this study can be a scaffold that can encapsulate bioactive material into its pore.