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    국내 경북지역 소에서 분리된 브루셀라 분리주의 생물학적 특성 = Biological characterization of Brucella spp. isolated from cattle in Gyeongbuk, Korea

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    https://www.riss.kr/link?id=A105746395

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    다국어 초록 (Multilingual Abstract) kakao i 다국어 번역

    Members of the genus Brucella are facultative intracellular bacteria and cause brucellosis, a chronic disease in humans and abortion in animals. In this study, we tested sera for brucellosis of 15 Hanwoo farms in the western part of Gyeong-buk province, resulting 5 farms were brucellosis positive in 2008. We collected blood from 277 heads in the brucellosis positive 5 farms, and performed serological diagnosis, brucella positive cattle which had shown higher than 200 antibody titer in tube agglutination test were slaughtered, supramammary lymph nodes were collected, and Brucella spp. wild type isolation and identification were performed. From these results, 15 of Brucella spp. wild type strains were isolated and all strains were identified as B. abortus biotype 1 by biological and molecular analysis. In the antimicrobial susceptibility test, all 15 strains had a similar susceptibility and resistance pattern. This study may be useful for bacteriological and epidemiological understanding of cattle brucellosis in Korea.
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    Members of the genus Brucella are facultative intracellular bacteria and cause brucellosis, a chronic disease in humans and abortion in animals. In this study, we tested sera for brucellosis of 15 Hanwoo farms in the western part of Gyeong-buk provinc...

    Members of the genus Brucella are facultative intracellular bacteria and cause brucellosis, a chronic disease in humans and abortion in animals. In this study, we tested sera for brucellosis of 15 Hanwoo farms in the western part of Gyeong-buk province, resulting 5 farms were brucellosis positive in 2008. We collected blood from 277 heads in the brucellosis positive 5 farms, and performed serological diagnosis, brucella positive cattle which had shown higher than 200 antibody titer in tube agglutination test were slaughtered, supramammary lymph nodes were collected, and Brucella spp. wild type isolation and identification were performed. From these results, 15 of Brucella spp. wild type strains were isolated and all strains were identified as B. abortus biotype 1 by biological and molecular analysis. In the antimicrobial susceptibility test, all 15 strains had a similar susceptibility and resistance pattern. This study may be useful for bacteriological and epidemiological understanding of cattle brucellosis in Korea.

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    참고문헌 (Reference)

    1 우종태, "홀스타인 유우로부터 Brucella abortus의 분리와 분리균의 성상에 관한 연구" 11 : 103-118, 1986

    2 김우택, "제주도내 축우 부루셀라병 발생상황 조사" 14 : 104-109, 1991

    3 김종만, "부루셀라 양성우에서 분리한 균의 성상과 혈청학적 진단법 비교" 30 : 1-6, 1988

    4 정종식, "경북지방 젖소로부터 Brucella abortus의 분리 및 균형별" 28 : 339-343, 1988

    5 심항섭, "경기도에서 발생하는 유우부루셀라병에 관한 연구. I. 감염우의 역학조사 및 분리균의 특성에 관하여" 19 : 189-198, 1996

    6 농림수산식품부, "결핵병 및 브루셀라병 방역 실시 요령" 농림수산식품부 2009

    7 Radostits OM, "Veterinary Medicine" Saunders 963-984, 2007

    8 Alton GG, "The serological diagnosis of bovine brucellosis: an evaluation of the complement fixation, serum agglutination and rose bengal tests" 51 : 57-63, 1975

    9 Alton GG, "Techniques for the Brucellosis Laboratory" Institut National de la Recherche Agronomique 1-156, 1988

    10 García-Rodríguez JA, "Susceptibilities of Brucella melitensis isolates to clinafloxacin and four other new fluoroquinolones" 39 : 1194-1195, 1995

    1 우종태, "홀스타인 유우로부터 Brucella abortus의 분리와 분리균의 성상에 관한 연구" 11 : 103-118, 1986

    2 김우택, "제주도내 축우 부루셀라병 발생상황 조사" 14 : 104-109, 1991

    3 김종만, "부루셀라 양성우에서 분리한 균의 성상과 혈청학적 진단법 비교" 30 : 1-6, 1988

    4 정종식, "경북지방 젖소로부터 Brucella abortus의 분리 및 균형별" 28 : 339-343, 1988

    5 심항섭, "경기도에서 발생하는 유우부루셀라병에 관한 연구. I. 감염우의 역학조사 및 분리균의 특성에 관하여" 19 : 189-198, 1996

    6 농림수산식품부, "결핵병 및 브루셀라병 방역 실시 요령" 농림수산식품부 2009

    7 Radostits OM, "Veterinary Medicine" Saunders 963-984, 2007

    8 Alton GG, "The serological diagnosis of bovine brucellosis: an evaluation of the complement fixation, serum agglutination and rose bengal tests" 51 : 57-63, 1975

    9 Alton GG, "Techniques for the Brucellosis Laboratory" Institut National de la Recherche Agronomique 1-156, 1988

    10 García-Rodríguez JA, "Susceptibilities of Brucella melitensis isolates to clinafloxacin and four other new fluoroquinolones" 39 : 1194-1195, 1995

    11 Morata P, "Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis" 36 : 2443-2446, 1998

    12 Romero C, "Specific detection of brucella DNA by PCR" 33 : 615-617, 1995

    13 Leal-klevezas DS, "Single-step PCR for detection of Brucella spp. from blood and milk of infected animals" 33 : 3087-3090, 1995

    14 Cloeckaert A, "Restriction site polymorphism of the genes encoding the major 25 kDa and 36 kDa outer-membrane proteins of Brucella" 141 : 2111-2121, 1995

    15 Fekete A, "Preliminary development of a diagnostic test for Brucella using polymerase chain reaction" 69 : 216-227, 1990

    16 Corbel MJ, "Methods for the Identification of Brucella" Central Veterinary Laboratory 4-35, 1978

    17 International Office of Epizootics, "Manual of Diagnostic Tests and Vaccines for Terrestrial Animals: Mammals, Birds and Bees" OIE 624-659, 2008

    18 Kim S, "Lipid raft microdomains mediate class A scavenger receptor-dependent infection of Brucella abortus" 37 : 11-19, 2004

    19 Alton GG, "Laboratory Techniques in Brucellosis" World Health Organization 1-163, 1975

    20 Herman L, "Identification of Brucella spp. by using the polymerase chain reaction" 58 : 2099-2101, 1992

    21 Lord VR, "Evaluation of humoral immunity to Brucella sp in cattle by use of an agar-gel immunodiffusion test containing a polysaccharide antigen" 50 : 1813-1816, 1989

    22 Nielsen K, "Enzyme-linked immunosorbent assay for differentiation of the antibody response of cattle naturally infected with Brucella abortus or vaccinated with strain 19" 50 : 5-9, 1989

    23 Bricker BJ, "Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51" 33 : 1640-1642, 1995

    24 Bricker BJ, "Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR" 32 : 2660-2666, 1994

    25 Corbel MJ, "Diagnostic Procedures for Non-Smooth Brucella Strains" Central Veterinary Laboratory 74-92, 1979

    26 Baily GG, "Detection of Brucella melitensis and Brucella abortus by DNA amplification" 95 : 271-275, 1992

    27 Cloeckaert A, "Classification of Brucella strains isolated from marine mammals by infrequent restriction site-PCR and development of specific PCR identification tests" 5 : 593-602, 2003

    28 López-Goñi I, "Brucella: Molecular and Cellular Biology" Horizon Bioscience 1-428, 2004

    29 Finegold SM, "Bailey and Scott’s Diagnostic Microbiology" C. V. Mosby 146-187, 1978

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