Porphyromonas gingivalis에 감염된 치주인대 섬유모세포의 유전자 발현 = Gene expression in primary periodontal ligament fibroblast responding to Porphyromanas gingivalis infection

다국어 초록 (Multilingual Abstract)

Periodontal ligament (PDL) cells are uniquely situated to maintain the overall integrity of the PDL, participating in repair, remodeling and regeneration of cememtum, alveolar bone and other supporting tissue of teeth. Fibroblasts (PDLF), the principal cells of the PDL, respond to bacteria, bacterial component, and soluble molecules secreted from neighboring cells, producing various inflammatory cytokines and other mediators which can cause inflammation of periodontal tissue and thus periodontal disease. The present study was performed to examine the change in gene expression of PDLF in response to periodontopathic Porphyromonas gingivalis. Primary PDLFs were incubated for 90 min with P. gingivalis 2561 pretreated with distilled water (DW-Pg) or 0.03% polyphosphate with a chain length of 65 (polyP-Pg) for 2 h. Invasion of PDLF by P. gingivalis during the period of time was measured by a metronidazole protection assay. DW-Pg was demonstrated to invade PDLF with the invasion efficiency of 0.0039%, while polyP-Pg was 0.0027%. cDNA microarray analysis was employed to identify genes that showed a greater than 1.5-fold difference in the gene expression of PDLF. The 90-min incubation with P. gingivalis increased the expression of proinflammatory chemokines/cytokines and their receptors including CCL3, CXCL1, CXCL3, CSF3, IL7R, TNFRSF11A (RANK) and IL1F5. In addition, TLR5 and apoptosis-related genes like NFKBIA, MAPK10, MAPK8IP2 and GAS2 were increased. Change of the expression by P. gingivalis was also found in genes of growth factors, transcription factors, arachidonic metabolism, coagulation, renin-angiotensin system, cell adhesion, cell-matrix interaction, cell communication, cell cycle, development and morphogenesis. Real-time PCR confirmed up-regulation of some selected genes. Among the genes upregulated in PDLF with DW-Pg, 5 genes including ADCY4, CAP2, EFNA3, MAPK8IP2 and one hypothetical proteins were decreased more than 1.5-fold in the gene expression of PDLF incubated with polyP-Pg.
Overall results suggest that P. gingivalis cause alteration of gene expression in PDLF, which may contribute to the development and progression of periodontal disease. polyP may be used for improving the disease.

목차 (Table of Contents)

• I. 서론
• II. 실험재료 및 방법
• III. 결과
• IV. 총괄 및 고찰
• V. 결론
• 참고문헌
• 영문초록