LPS로 유도된 RAW 264.7 대식세포에 대한 애기외톨개 모자반 (Myagropsis yendoi) 에틸아세테이트 분획물의 항염증 효과 = Anti-inflammatory Effect of an Ethyl Acetate Fraction from Myagropsis yendoi on Lipopolysaccharides-stimulated RAW 264.7 Cells

다국어 초록 (Multilingual Abstract)

An ethanolic extract from Myagropsis yendoi was fractionated using several solvents. Among these, an ethyl acetate fraction (Myagropsis yendoi ethyl acetate fraction: MYE) showed the highest anti-inflammatory activity based on inhibition of lipopolysaccharides (LPS)-induced nitric oxide (NO) production in RAW 264.7 cells. We thus investi-gated the molecular mechanisms underlying MYE’s inhibitory effects. Pretreatment of cells with up to 30 μg/mL of MYE significantly inhibited NO production and inducible nitric oxide synthase expression in a dose-dependent man-ner (P<0.05). Similarly, MYE markedly reduced the production of pro-inflammatory cytokines, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α, as well as their mRNA levels. While the nuclear translocation of nuclear factor-kappa B (NF-κB) was strongly suppressed by MYE, the activation of a nuclear factor erythroid 2-re-lated factor (Nrf2) was increased. Moreover, MYE significantly reduced the phosphorylation of JNK, p38 MAPK, and phosphatidylinositol 3-kinase/Akt in LPS-stimulated cells. These results indicate that MYE contains anti-inflam-matory compounds, and that it might be used as a dietary supplement for the prevention of inflammatory diseases.

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