pH, 무기염류, 생장조절제 및 광 조건이 차나무의 기내 배양에 미치는 영향 = Effect of pH, Inorganic Salt, Plant Growth Regulators and Conditoin of Light on In Vitro Masspropagation of Camellia sinensis 〔L.〕 O. Kuntze

국문 초록 (Abstract)

우리는 기내 유식물의 single node를 이용하여 수소이온 동도, 염류농도, 광조건별로 증식배양하여 차나무 유식물에 미치는 영향을 조사분석 하였으며 재래종과 품종 명선의 효율적인 대량증식 방법을 찾았다. 수소이온농도가 차나무 증식에 미치는 영향은 pH가 산성일 경우(pH 3.5) 생존율이 20%로 낮았고 배지가 굳지 않았다. 적정 pH는 4.5-5.5로 생존율과 생육이 가장 양호하였다. 무기염류농도는 MS 배지1배에서 신초생장 및 증식이 가장 양호하였다. 광조건은 16시간 연속 명배양, 8시간 암배양이 식물의 생장과 증식에 가장 양호하였다. 24시간 명배양 하였을 경우 잎의 노화현상이 발생하였으며 줄기가 경화하였고, 24시간 암배양 하였을 경우에는 생육이 부진하였다. 다아체의 발생은 MS배지에 TDZ 0.005 ㎎/L를 첨가한 처리에서 증식효율이 가장 높게 나타났다. 이 방법은 차나무 품종 및 희귀종의 급속 대량증식에 적용 가능 하며 교배육종 시 육종기간 단축을 위한 조기 품종특성 평가 방법으로 활용이 가능할 것이다.

다국어 초록 (Multilingual Abstract)

In order to investigate effective mass propagation condition, single node of in vitro Camellia sinensis (L.) O. Kuntze cv. Moungsun and native kind were cultured by using some kinds of modified tissue culture condition. In effect that hydrogen ion concentration gets in tea propagation, media did not harden and early survival rate was low by 20% in pH 3.5. optimum pH was 4.5 to 5.5, in these condition survival rate and growth was most superior. In the case of micronutrient concentration condition, shoot growth and propagation were the best in MS media standard concentration. In photoperiod’s effect about tea organogenesis, continued lighting for 16 hours and dark culture for 8 hours was the best condition in growth and propagation of plant. Aging symptoms of plant appeared in case of light culture for 24 hours, and plant growth was in exhaustible in case of dark culture for 24 hours. In result of cytokinins treatment for propagation, growth efficiency appeared highest in thidiazuron (TDZ) 0.005 ㎎/L. It was expected that these results are used at Masspropagation of Camellia sinensis (L.) O. Kuntze, and characterizing the varieties at crossing inbreed.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 적요
• 참고문헌

참고문헌 (Reference)

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