식방풍의 성분분리 및 생리활성 = Biolosical Activities of Isolated Compounds from Peucedani Radix

다국어 초록 (Multilingual Abstract)

In this study, isolation of antioxidative compounds was performed for development of anti-oxidizing agent. $CHCl_3$, $H_2O$, 30%, 60% MeOH, MeOH fractions were examined antioxidative activity by DPPH, test of inhibition on NO production. It was revealed that 30%, 60% MeOH and $CHCl_3$ fractions had significant antioxidative activity. In 30% MeOH and 60% MeOH, $CHCl_3$ fraction, six compounds were isolated and elucidated as adenosine(I), guanosine(II), peucedanol 7-O-$\beta$-D-apiofuranosyl(1$\rightarrow$6)-$\beta$-glucopyranoside(III), peucedanol 7-O-$\beta$-D-glucopyranoside(IV), peucedanol(V) and scopoletin(VI) by physicochemical data and spectroscopic methods. (Negative FAB-MS, $^{1}H-NMR$, $^{13}C-NMR$). The results from antioxidative activity screening for the each compound showed that compound IV was relatively superior antioxidant ability. In anti-inflammatory activation assay, compound III, IV, VI had concentration-dependent-activity and compound IV had superior anti-inflammatory ability. These results suggest that Peucedani Radix might be developed as a potent anti-oxidative, anti-inflammatory agents and ingredients for related functional foods.

참고문헌 (Reference)

1 Hata, Y, 88 : 513-, 1968

2 한국한의학연구원, "한약재 관능검사기준 연구(한약재도감편)" 보건복지부 88-89, 1999

3 김호철, "한약약리학" 집문당 75-, 2004

4 생약학교재편찬위원회, "생약학" 동명사 137-, 2006

5 "대한 약전 제 8개정 대한 약전외 한약(생약) 규격집"

6 Denisa, D, "β-Adenosine, a bioactive compound in grass chaff stimulating mushroom production" 65 : 181-, 2004

7 Morioka, T, "The modifying effect of Peucedanum japonicum, a herb in the Ryukyu Islands, on azoxymethane-induced colon preneoplastic lesions in male F344 rats" 205 (205): 133-, 2004

8 KiHwan Bae, "The Constituents Isolated from Peucedanum japonicum Thunb. and their Cyclooxygenase (COX) Inhibitory Activity" 한국약용작물학회 13 (13): 75-79, 2005

9 Whang, W. K., "Standardization of Peucedani Radix" 32 (32): 292-, 2001

10 Mosmann, T, "Rapid colormetric assay for the cellular growth and survival" 65 : 55-, 1983

11 Shin, K. H, "Quantitative analysis of peucedanol in Peucedanum japonicum" 23 (23): 1992

12 Takeuchi, N, "Pharmacological activities of the prenylcoumarins, developed from folk usage as a medicine of Peucedanum japonicum thunb" 39 (39): 1415-, 1991

13 Aida, Y, "Pharmacological activities of khellactones, compounds isolated from Peucedanum japonicum thunb. and Peucedanum raeruptorium DUNN" 20 (20): 343-, 1998

14 Feelisch, M, "Methods in nitric oxide research" John Wiley & Sons 492-297, 1983

15 Lee, J. W, "Mechanisms of relaxant action of a pyranocoumarin from Peucedanum japonicum in isolated rat thoracic aorta" 68 (68): 891-, 2002

16 Huong, D. T, "Inhibitory activity of monoamine oxidase by coumarins from Peucedanum japonicum" 22 (22): 324-, 1999

17 So Young Park, "Inhibitory Constituents of LPS-induced Nitric Oxide Production from Arctium lappa" 한국생약학회 11 (11): 85-88, 2005

18 Choi, H. C, "Inhibition of nitric oxide production by coumarins from Peucedanum Japonicum in LPS-Activated RAW 264.7 cells" 30 (30): 99-, 1999

19 Hatano, T, "Effects of the interaction of tannins with co-existing substances. IV. Effects of tannins and related polyphenols on superoxide anion radical, and on 1,1-diphenyl-2-picrylhydrazyl radical" 37 : 2016-, 1989

20 Yasumasa, I, "Dihydropyranocoumarins from roots of Peucedanum japonicum" 31 (31): 4303-, 1992

21 Yasumasa, I, "Dihydropyranocoumarins from Peucedanum japonicum" 33 (33): 1543-, 1993

22 Chang, Y. D, "Cytotoxic pyranocoumarins from the aerial parts of Peucedanum japonicum" 30 (30): 2812-, 1991

23 Chang, Y. D, "Cytotoxic pyranocoumarins from roots of Peucedanum japonicum" 31 (31): 1829-, 1992

24 Ikeshiro, Y, "Coumarins glycosides from Peucedanum japonicum" 35 (35): 1339-, 1994

25 Teijeira, M, "Assignment of the 13C NMR spectra of some adenine, hypoxanthine and guanine carbonucleosides" 35 : 806-, 1997

26 Lee, S. O., "Antidiabetic coumarin and cyclitol compounds from Peucedanum japonicum" 27 (27): 1207-, 2004

27 Jong, T. T, "An antiplatelet aggregation principle and X-ray structural analysis of cis-khellactone diester from Peucedanum japonicum" 55 (55): 1396-, 1992